Detailed information of TA system TA147428

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 936562..937241
• Replicon: chromosome
• Accession: NZ_CP047729
• Organism: Enterobacter hormaechei strain Eho-6

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2J0PMI2
• Locus tag: GVI67_RS04450
• Protein ID: WP_039273191.1
• Coordinates: 936562..936903 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2J0PNI1
• Locus tag: GVI67_RS04455
• Protein ID: WP_039273193.1
• Coordinates: 936924..937241 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI67_RS04430	932554..933657	+	1104	WP_006809995.1	glutamate 5-kinase	-
GVI67_RS04435	933669..934922	+	1254	WP_006809994.1	glutamate-5-semialdehyde dehydrogenase	-
GVI67_RS04445	935516..936484	+	969	WP_000654811.1	IS5 family transposase	-
GVI67_RS04450	936562..936903	-	342	WP_039273191.1	TA system toxin CbtA family protein	Toxin
GVI67_RS04455	936924..937241	-	318	WP_039273193.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GVI67_RS04460	937261..937482	-	222	WP_039273195.1	DUF987 domain-containing protein	-
GVI67_RS04465	937491..937967	-	477	WP_039273197.1	RadC family protein	-
GVI67_RS04470	937983..938444	-	462	Protein_861	antirestriction protein	-
GVI67_RS04475	938535..938704	-	170	Protein_862	DUF905 family protein	-
GVI67_RS04480	939188..941185	+	1998	WP_039274705.1	choline BCCT transporter BetT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	935561..988036	52475
	-	flank	IS/Tn	-	-	935561..936484	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12851.82 Da
• Isoelectric Point: 9.6545

>T147428 WP_039273191.1 NZ_CP047729:c936903-936562 [Enterobacter hormaechei]
MKPLPATTSRAAKPCLSPVAVWQILLTHLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRRGF
SRQEQSPYLRAVDILRARQVTGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T147428 NZ_CP062984:c1215676-1214930 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTATCAGATCATGGTGTGGACAATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCCTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGACACGCGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGTAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11890.58 Da
• Isoelectric Point: 7.7498

>AT147428 WP_039273193.1 NZ_CP047729:c937241-936924 [Enterobacter hormaechei]
MSNITWGLQRNITPRLGARLVQDRNRMHYLADRASITGKFSDAECLKLDVAFPHFISQMESMLTTGELNPRHPHCIALYH
NGFTCEADTLGSCGYVYIAVYPSQR

Nucleotide

• Length: 318 bp

>AT147428 NZ_CP062984:c1214930-1214760 [Bacillus amyloliquefaciens]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2J0PMI2

Antitoxin

• AlphaFold DB: A0A2J0PNI1