Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1684910..1685707 Replicon chromosome
Accession NZ_CP047715
Organism Enterobacter hormaechei strain Eho-E2

Toxin (Protein)


Gene name KacT Uniprot ID F5RT91
Locus tag GVI74_RS08015 Protein ID WP_006809321.1
Coordinates 1685186..1685707 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag GVI74_RS08010 Protein ID WP_001303459.1
Coordinates 1684910..1685179 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI74_RS07980 1680532..1681032 + 501 WP_032673548.1 DedA family protein -
GVI74_RS07985 1681137..1682378 + 1242 WP_006809325.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
GVI74_RS07990 1682411..1682638 - 228 WP_006809324.1 YccJ family protein -
GVI74_RS07995 1682657..1683253 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
GVI74_RS08000 1683644..1683814 + 171 WP_001273664.1 general stress protein -
GVI74_RS08005 1683931..1684836 + 906 WP_039273532.1 DMT family transporter -
GVI74_RS08010 1684910..1685179 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
GVI74_RS08015 1685186..1685707 + 522 WP_006809321.1 GNAT family N-acetyltransferase Toxin
GVI74_RS08020 1685766..1687088 - 1323 WP_006809320.1 pyrimidine utilization transport protein G -
GVI74_RS08025 1687110..1687604 - 495 WP_006809319.1 pyrimidine utilization flavin reductase protein F -
GVI74_RS08030 1687614..1688204 - 591 WP_006809318.1 malonic semialdehyde reductase -
GVI74_RS08035 1688214..1689014 - 801 WP_006809317.1 pyrimidine utilization protein D -
GVI74_RS08040 1689022..1689408 - 387 WP_006809316.1 pyrimidine utilization protein C -
GVI74_RS08045 1689420..1690109 - 690 WP_006809315.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19494.28 Da        Isoelectric Point: 7.9346

>T147413 WP_006809321.1 NZ_CP047715:1685186-1685707 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGNFDCGEPSLNAFLTDHLVRQHSGRILRGYLLKERDHPRILGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQV

Download         Length: 522 bp

>T147413 NZ_CP062951:c1415749-1415465 [Bifidobacterium longum subsp. infantis]
ATGAGCACCGAATTCACCGACTACGACACCAGCGAATACCTCGAAAACGAACAGGACATCATCGCCTACCTCAACGCCAT
AGCCGAATACGACGACCCCGCACTCATGCAGGCCGCGCTCGGCAACGTCGCCAAGTCCCGGGGCATGACCCAGATCGCCA
AGGACGCGGGCGTGGGGCGCGAAAGCCTCTACAAGAGCCTCAGCAAGGACGGCAACCCCAGCTTCCAGACCATCGCCAAG
GTAATCCACGCCCTCGGCGGACGCCTCACCATCCAAGCCGCCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT147413 WP_001303459.1 NZ_CP047715:1684910-1685179 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT147413 NZ_CP062951:c1416054-1415746 [Bifidobacterium longum subsp. infantis]
GTGGAAATCAAGCAATCCGCCGAATACCGCAAGTGGTTCAAGAAACTCAGGGACCACAAAGCGAAGGCCGCCATCCAAGC
CCGGCTCGATGCCTGCAAGCTCGCAGGCAGGCCATTCGGCGGCATCAAACCCGTGGAAGGCCCGGTCAGCGAGATGCGGT
TCCACACCGGGGCCGGATACCGCGTCTACTTCGCCATGCAGGGCAACGTGCTCATGCTGCTGCTCGCAGGCGGCGACAAA
AGCACCCAGCAGACCGACATCAGACAAGCCCACGACATCCTCAACGACTACAAGGAACAACGACAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1R089

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References