Detailed information of TA system TA147411

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 936527..937206
• Replicon: chromosome
• Accession: NZ_CP047715
• Organism: Enterobacter hormaechei strain Eho-E2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2J0PMI2
• Locus tag: GVI74_RS04455
• Protein ID: WP_039273191.1
• Coordinates: 936527..936868 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2J0PNI1
• Locus tag: GVI74_RS04460
• Protein ID: WP_039273193.1
• Coordinates: 936889..937206 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI74_RS04435	932519..933622	+	1104	WP_006809995.1	glutamate 5-kinase	-
GVI74_RS04440	933634..934887	+	1254	WP_006809994.1	glutamate-5-semialdehyde dehydrogenase	-
GVI74_RS04450	935481..936449	+	969	WP_000654811.1	IS5 family transposase	-
GVI74_RS04455	936527..936868	-	342	WP_039273191.1	TA system toxin CbtA family protein	Toxin
GVI74_RS04460	936889..937206	-	318	WP_039273193.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GVI74_RS04465	937226..937447	-	222	WP_039273195.1	DUF987 domain-containing protein	-
GVI74_RS04470	937456..937932	-	477	WP_039273197.1	RadC family protein	-
GVI74_RS04475	937948..938409	-	462	Protein_860	antirestriction protein	-
GVI74_RS04480	938500..938669	-	170	Protein_861	DUF905 family protein	-
GVI74_RS04485	939153..941150	+	1998	WP_039274705.1	choline BCCT transporter BetT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	935526..988001	52475
	-	flank	IS/Tn	-	-	935526..936449	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12851.82 Da
• Isoelectric Point: 9.6545

>T147411 WP_039273191.1 NZ_CP047715:c936868-936527 [Enterobacter hormaechei]
MKPLPATTSRAAKPCLSPVAVWQILLTHLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDRRGF
SRQEQSPYLRAVDILRARQVTGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T147411 NZ_CP062950:391682-392173 [Bifidobacterium longum subsp. longum]
ATGAGGCAGTCAAAGCAGGTGTCGATCTTGTGCGACACCAATATCCTTATTTATTACATGCTCTGTACGCCTCGGTTCAT
CAAGGATGTCATGAAACTGTTCGAGCGATGCGTCGAGCGAGAGGTAAAGCTGTCCTGTGCGTCACTGTCGCTGAAGGACG
TGTTCTACATCACCCCGGAACGATTCAAAGAACGATTCATGAATGACGAATCCATGGTCGTTGCGACCACCGCCAAGCTG
ATATTGCCGGAGGTGGCATGGTCGGGAATCAGCAAACTCAAGGAGCTCATGACAATCGTGCCGATTGGCGCGGAGGAATG
TGAATTGGCGCAGTCGTTGCGGCACGCGCATAACGATTTCGAGGATGACCTTATTATCGCCGCCGCACAACAATCCGGGG
CCACCTATGTGGTGACGTATGACAAAAAACTAGCCAAGCATTTTCCCGAATATTGCAAAACGCCGGCTGAAATCGTGGCG
GCGCTGGCATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11890.58 Da
• Isoelectric Point: 7.7498

>AT147411 WP_039273193.1 NZ_CP047715:c937206-936889 [Enterobacter hormaechei]
MSNITWGLQRNITPRLGARLVQDRNRMHYLADRASITGKFSDAECLKLDVAFPHFISQMESMLTTGELNPRHPHCIALYH
NGFTCEADTLGSCGYVYIAVYPSQR

Nucleotide

• Length: 318 bp

>AT147411 NZ_CP062950:391293-391685 [Bifidobacterium longum subsp. longum]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTGTACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAAGCCCGCGAGGAGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTACACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGATGAGATAGCTGA
CGAACGGCTGAAGGAATACAGGCATCAGTCGGACTATGCGCGTTATTTGGAGAAGGAGCGGAGTCTGTCATGA

Structures

Toxin

• AlphaFold DB: A0A2J0PMI2

Antitoxin

• AlphaFold DB: A0A2J0PNI1