Detailed information of TA system TA147409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 91480..93048
• Replicon: plasmid pEco15-1
• Accession: NZ_CP047711
• Organism: Escherichia coli strain Eco-15

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A859R327
• Locus tag: GVI77_RS23255
• Protein ID: WP_024134513.1
• Coordinates: 91741..93048 (+)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A859R2S1
• Locus tag: GVI77_RS23250
• Protein ID: WP_039272366.1
• Coordinates: 91480..91731 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI77_RS23220	87133..87858	+	726	WP_001553093.1	hypothetical protein	-
GVI77_RS23225	87914..88366	+	453	WP_001553092.1	hypothetical protein	-
GVI77_RS23230	88382..88984	-	603	WP_039272372.1	hypothetical protein	-
GVI77_RS23235	89245..89526	+	282	WP_000575656.1	hypothetical protein	-
GVI77_RS23240	89545..90108	+	564	WP_001132404.1	hypothetical protein	-
GVI77_RS23245	90111..91115	+	1005	WP_039272368.1	tyrosine-type recombinase/integrase	-
GVI77_RS23250	91480..91731	+	252	WP_039272366.1	helix-turn-helix domain-containing protein	Antitoxin
GVI77_RS23255	91741..93048	+	1308	WP_024134513.1	type II toxin-antitoxin system HipA family toxin	Toxin
GVI77_RS23260	93045..93539	+	495	WP_039272364.1	hypothetical protein	-
GVI77_RS23265	93714..94652	-	939	WP_044596315.1	hypothetical protein	-
GVI77_RS23270	94851..96491	+	1641	WP_031611419.1	PAAR domain-containing protein	-
GVI77_RS23275	96863..97165	-	303	WP_031611417.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / mph(A) / blaSHV-12 / blaVIM-1 / aph(3')-XV / ant(3'')-Ia / catB2 / qacE / sul1	-	1..152546	152546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-64)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 47950.12 Da
• Isoelectric Point: 6.2444

>T147409 WP_024134513.1 NZ_CP047711:91741-93048 [Escherichia coli]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Nucleotide

• Length: 1308 bp

>T147409 NZ_CP062949:c1461404-1461069 [Bifidobacterium longum subsp. longum]
ATGCTCAAACCGGATTACACGGCGGCATTCCAGCGAGATATCAAGAAGCTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTACTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTTGGGCAAATAGCGGGTTTACATCCTGCTTTGAACCA
GCCGACGGCGATTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9369.79 Da
• Isoelectric Point: 9.8930

>AT147409 WP_039272366.1 NZ_CP047711:91480-91731 [Escherichia coli]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Nucleotide

• Length: 252 bp

>AT147409 NZ_CP062949:c1461677-1461408 [Bifidobacterium longum subsp. longum]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAAGCGCATTCCCCTAAACCTTTCCTATACGA
TCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCCAACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Toxin

• AlphaFold DB: A0A859R327

Antitoxin

• AlphaFold DB: A0A859R2S1