Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4231808..4232305 Replicon chromosome
Accession NZ_CP047710
Organism Escherichia coli strain Eco-15

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GVI77_RS20255 Protein ID WP_001173343.1
Coordinates 4231808..4231981 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag GVI77_RS20260 Protein ID WP_001312331.1
Coordinates 4232009..4232305 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI77_RS20245 4228478..4229935 + 1458 WP_001610404.1 dipeptide/tripeptide permease DtpC -
GVI77_RS20250 4230172..4231689 + 1518 WP_000003806.1 lysine--tRNA ligase -
GVI77_RS20255 4231808..4231981 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GVI77_RS20260 4232009..4232305 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GVI77_RS20265 4232533..4232805 - 273 WP_000405643.1 N-acetyltransferase -
GVI77_RS20270 4233019..4236948 - 3930 WP_001080127.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4231808..4247988 16180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T147408 WP_001173343.1 NZ_CP047710:c4231981-4231808 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T147408 NZ_CP062949:c1140328-1140074 [Bifidobacterium longum subsp. longum]
ATGCTGCTCTGCTGGACCGAAGACGCCTGGGCCGACTACCTGTACTGGCAGGCTCAGGACCGCAAGACCCTCAAGCGCGT
CAACACGCTCATCCGGGACATGCAGCGAACCCCGTTCGAAGGCATCGGCAAGCCCGAACCCCTCAAATGGGGCCTGTCCG
GCGCATGGTCGCGGCGTATCGATTCGGCCAACCGTATCATCTATACCGTCGCCGACGACAGGCTCTGCATCCTCTCCGCG
AAGGACCACTACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT147408 WP_001312331.1 NZ_CP047710:c4232305-4232009 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT147408 NZ_CP062949:c1140594-1140328 [Bifidobacterium longum subsp. longum]
ATGACACAGGCCGTGGCCTACAGCAATTTCAGGGCGAATCTCAAGACCTATATGCGCAAGGTCAACGAGGACGCCGACAC
CCTTCTCGTGACCAACGCGGACCCGGAGGACAACGTGGTCGTGATGAGCGCCGACGACTACGATTCGCTCATGGAGACGC
TGCGCGTCTACCAGAACCCGTATCTAAGCGACAAGGTGATGCGCGGCATGGCGCAGGTCCGGCAGGGCCAGACGATGGCC
CATGACCTTATCGAAGCGGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References