Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3842857..3843407 Replicon chromosome
Accession NZ_CP047710
Organism Escherichia coli strain Eco-15

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag GVI77_RS18345 Protein ID WP_001160966.1
Coordinates 3842857..3843171 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag GVI77_RS18350 Protein ID WP_000125566.1
Coordinates 3843174..3843407 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI77_RS18320 3838532..3839818 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
GVI77_RS18325 3839818..3840807 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GVI77_RS18330 3840804..3841625 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GVI77_RS18335 3841628..3842005 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GVI77_RS18340 3842012..3842860 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GVI77_RS18345 3842857..3843171 - 315 WP_001160966.1 CcdB family protein Toxin
GVI77_RS18350 3843174..3843407 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GVI77_RS18355 3843493..3843972 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
GVI77_RS18360 3844164..3846026 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
GVI77_RS18365 3846019..3846549 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GVI77_RS18370 3846657..3847988 - 1332 WP_001183207.1 MFS transporter -
GVI77_RS18375 3848047..3848334 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T147404 WP_001160966.1 NZ_CP047710:c3843171-3842857 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T147404 NZ_CP062946:c2628476-2628189 [Thalassotalea sp. LPB0316]
ATGAGTTATGAACTGGAATTTAAAAAGTCAGCTCTGAAAGAGTGGAAAAAACTTGGCGCCACTATTCAAACTCAGTTTAA
AAAGAAGTTAGCAGAAATTTTAGTTAATCCACACATACCAAGTGCAAAGTTATCTGGCGCCAATGAACTCTATAAAATAA
AGCTACGACAAGCTGGTTACCGACTAGTTTATGAAGTAAACGATGAAATAATTACAGTAACCGTAATCTCTGTAGGTAAG
CGAGACAAAGGAAAAGTCTATAAAGTTGCGATGAAACGCAATGATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT147404 WP_000125566.1 NZ_CP047710:c3843407-3843174 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT147404 NZ_CP062946:c2628714-2628466 [Thalassotalea sp. LPB0316]
ATGACTACTAGGATTTTAACAGAAACAGCAGCAAGTATTAGTGAATTAAAAGCTAACCCCATGAAAGTTGCTTTAAGCGC
AAATGGCGAACCGATCGCTATATTAAATAGAAATGAGCCAGCGTTCTATTGCATACCTGCAGAAACGTATGAATTTTTAA
TGGATCATTTAGAAGATTTAGAGCTAATGCAAATTGCCGAAAGCAGAAAAAATGAAGAAAGCATTAAGGTTAGCCTAGAT
GAGTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References