Detailed information of TA system TA147403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3613323..3615012
• Replicon: chromosome
• Accession: NZ_CP047710
• Organism: Escherichia coli strain Eco-15

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: GVI77_RS17275
• Protein ID: WP_000365807.1
• Coordinates: 3613323..3614243 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: GVI77_RS17280
• Protein ID: WP_001295795.1
• Coordinates: 3614230..3615012 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI77_RS17235	3608796..3609506	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
GVI77_RS17240	3609813..3610142	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
GVI77_RS17245	3610154..3610317	-	164	Protein_3378	tail fiber assembly protein	-
GVI77_RS17250	3610391..3611005	-	615	WP_001019923.1	YagU family protein	-
GVI77_RS17255	3611220..3611405	-	186	WP_000146240.1	hypothetical protein	-
GVI77_RS17260	3611454..3611612	+	159	Protein_3381	integrase	-
GVI77_RS17265	3611839..3612174	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
GVI77_RS17270	3612382..3613158	+	777	WP_000171155.1	site-specific integrase	-
GVI77_RS17275	3613323..3614243	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GVI77_RS17280	3614230..3615012	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
GVI77_RS17285	3615296..3618394	+	3099	WP_000673070.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3572334..3621262	48928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T147403 WP_000365807.1 NZ_CP047710:c3614243-3613323 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T147403 NZ_CP062946:2572400-2572663 [Thalassotalea sp. LPB0316]
ATGACTAATCGTTTACTTTCTTGGACGGACGAGGCTTGGGATCCTTATCTATATTGGCAAACACAAGATAATAAAACGCT
GAAGCGTATCAATAAACTGGTTACTGATGTTAAGCGTTCACCTTTTGAAGGCATTGGTAAACTAGAGCCTTTAAAAGAAA
ATTTAACTAGCTTTTGGTCTAGCCGAATAGATGATACCAATCGTTTAGTTAATGCCGTTGATGATCATGCGATAACAATA
ATTTCGTGTCATTATCATTACTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT147403 WP_001295795.1 NZ_CP047710:c3615012-3614230 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT147403 NZ_CP062946:2572153-2572407 [Thalassotalea sp. LPB0316]
ATGAGAATAGTATCGTTCACAGAAGCAAGAAATAGTTTAAAAGCAGTGCTAGATGGTATCGTTAATGACGCTGACACAAC
AGCGATCACGCGTAGAGACTCTGAAGATGCAATTGTTATGTCATTAGACTATTACAACAGTCTGATGGAAACCGTGCACC
TGCTCCGTTCACCAGCAAATACTGAACACTTGAATCGTTCGATATCACAATATAAGTCAGGTAAGGTAAATCACAGAGAC
TTAATTGATGACTAA

Structures

Toxin

• AlphaFold DB: A0A061KTE0