Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 292757..293557 Replicon chromosome
Accession NZ_CP047710
Organism Escherichia coli strain Eco-15

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag GVI77_RS01340 Protein ID WP_000342452.1
Coordinates 293030..293557 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag GVI77_RS01335 Protein ID WP_001277107.1
Coordinates 292757..293023 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI77_RS01315 288416..289084 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
GVI77_RS01320 289077..290135 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
GVI77_RS01325 290380..291234 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GVI77_RS01330 291505..292608 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GVI77_RS01335 292757..293023 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
GVI77_RS01340 293030..293557 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
GVI77_RS01345 293554..293937 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
GVI77_RS01350 294360..295469 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GVI77_RS01355 295517..296443 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GVI77_RS01360 296440..297717 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
GVI77_RS01365 297714..298481 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T147385 WP_000342452.1 NZ_CP047710:293030-293557 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T147385 NZ_CP062939:c2497346-2497056 [Bifidobacterium subtile]
ATGCTTGTTCCCGAATACACAACTGGCTTTGAGCGGGATGTGAAGCGAATGACCAGGAAGCATGTGAACACCGAGGGCCT
GCGCCGGGTCATCCGTCTGGTGCTTGCAGACGACGAGCAGTCGCGCGAGGAGCTGCGGCGCAGGCATCGTGCCCATAGGC
TCACTGGCGACTGGAGCGGGGTGCTCGAGTGCCACATCGACAACAGTGCCGATAGTCTGCTGATATGGCGCACGGCCAGC
GGCACTGCTGTCTTCCTTCGCGTGGGCAGCCATGATGAGCTGTTCCAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT147385 WP_001277107.1 NZ_CP047710:292757-293023 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT147385 NZ_CP062939:c2497615-2497349 [Bifidobacterium subtile]
ATGACAACGGTTACCGTGCGAGTGGACGATGAGACCAAGCTGAAGGCCTCCAATATTGCAGAGGATTTCGGCTTCGATCT
TTCCTCTGTTACCAGGGCGTTTTATCGCCAGATGGTGCGGGAACGTCGCATTCCGCTGAACCTCAGCTACGAGCCGACGC
CTAATGAGCAGACTCTCGCCGCGATTCATGAGGCGGAGGAGATAGCGCAGACTGGCCGTCAGCGATTCAAGAACGCTGAT
GAGATGTTCGATTCGCTGGAAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References