Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 10018..10661 Replicon plasmid pKpn13-2
Accession NZ_CP047709
Organism Klebsiella pneumoniae strain Kpn-13

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GVI78_RS26175 Protein ID WP_000754566.1
Coordinates 10018..10434 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GVI78_RS26180 Protein ID WP_001261276.1
Coordinates 10431..10661 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI78_RS26150 5130..5894 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -
GVI78_RS26155 6387..6971 - 585 WP_001137892.1 TetR family transcriptional regulator -
GVI78_RS26160 6971..8209 - 1239 WP_000004159.1 MFS transporter -
GVI78_RS26165 8206..9111 - 906 WP_000219391.1 Mph(A) family macrolide 2'-phosphotransferase -
GVI78_RS26170 9233..9937 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
GVI78_RS26175 10018..10434 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI78_RS26180 10431..10661 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GVI78_RS26185 11250..11543 + 294 WP_020956883.1 hypothetical protein -
GVI78_RS26190 11607..12293 + 687 WP_020956882.1 hypothetical protein -
GVI78_RS26195 12305..13081 + 777 WP_020956881.1 site-specific integrase -
GVI78_RS26205 14866..14970 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / mph(A) / blaVIM-1 / ant(3'')-Ia / qacE / sul1 / qnrS1 - 1..81960 81960
- inside IScluster/Tn dfrA14 / mph(A) - 4027..14577 10550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T147383 WP_000754566.1 NZ_CP047709:c10434-10018 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T147383 NZ_CP062939:c1649172-1648858 [Bifidobacterium subtile]
ATGACGAGCTCTAGGCCCTTGCCCGTAATCGTCATTGGGCAGGTCCGATACCAGCTCACCCGGCATGTGCTGTGGGCGAG
CGGAGTCCATGACGACTACCTCGAATGGAGGAGAACCGACGCTAAACGATTCGAGCGCATTCAGCGCCTTATTGTGGACA
TCGACCAGCATCCATTCAGCGGGCTGGGCAAGCCCGAACCCCTGCGACACAATCTCGCTGGTGCATGGAGTCGGCATGTC
AGGCAGGAGCATCGACTCATCTATCACGTCGACAATCAAGGCATCCACTTGTACTCAGTGCGGGATCATTACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT147383 WP_001261276.1 NZ_CP047709:c10661-10431 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT147383 NZ_CP062939:c1649410-1649159 [Bifidobacterium subtile]
ATGCTTACTCTGCCGTACACATCTTTTCGCAATAATATGAAGGAGAACATGCGCAGGCTTCATGACGATGCCGACGTGAT
GCTCGTTACCAACCGCGATCCGGCGGACAATATCGTCGTCATGAGTCAGGATGACTACGAATCGCTTATGGAGACGCTGC
GCGTGCAGTCGAATCCTGATCTGCATCGCCGCGTCTTGGCTGGCATGCGCCAGAGTGCCGAGGGCGCCGAGGAGGAGTAT
GACGAGCTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References