Detailed information of TA system TA147382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 72450..73093
• Replicon: plasmid pKpn13-1
• Accession: NZ_CP047708
• Organism: Klebsiella pneumoniae strain Kpn-13

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: GVI78_RS25515
• Protein ID: WP_193125710.1
• Coordinates: 72450..72866 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: GVI78_RS25520
• Protein ID: WP_001261276.1
• Coordinates: 72863..73093 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI78_RS25500	68408..69105	+	698	Protein_66	IS1 family transposase	-
GVI78_RS25505	69169..70692	-	1524	WP_193125711.1	EAL domain-containing protein	-
GVI78_RS25510	71127..72355	-	1229	Protein_68	IS3 family transposase	-
GVI78_RS25515	72450..72866	-	417	WP_193125710.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI78_RS25520	72863..73093	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GVI78_RS25525	73587..74035	+	449	Protein_71	hypothetical protein	-
GVI78_RS25530	74260..75267	-	1008	WP_065808204.1	alpha/beta hydrolase	-
GVI78_RS25535	75484..76251	-	768	WP_193125709.1	alpha/beta hydrolase	-
GVI78_RS25540	76404..76871	+	468	WP_064175061.1	MarR family transcriptional regulator	-
GVI78_RS25545	76922..76978	+	57	WP_003032489.1	hypothetical protein	-
GVI78_RS25550	77176..77394	+	219	WP_064158655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..181030	181030

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15120.66 Da
• Isoelectric Point: 8.5403

>T147382 WP_193125710.1 NZ_CP047708:c72866-72450 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRV
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T147382 NZ_CP062938:1359965-1360324 [Bifidobacterium eulemuris]
ATGTGGAACATAGTGTTGGACCCAATCAAAGACTGGCTGGACACTCTAGATACCGAATCCGCGGAACAAGTGCAAGCCGC
GCTTGAAATACTTCGCGACCAAGGGCCGAATCTCAAGCGCCCACTAGTCGGCAAGATTGAGGGTTCGCGCATCGTCAAAT
CAATGAAAGAATTACGTCCCGGCTCTGTAGGTCGGAGCGAGATACGCATTCTATTCGTCTTCGACCCCAAACGACAGGCA
ATCATGCTGGTCGGCGGCGACAAACAACGCAGATGGAACAAATGGTACAAAACAGCCATCGAGCAGGCTGAGGCACGTTA
TCTGGCGTGGCTTGAAGAACAATACAGCAAGGAGAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT147382 WP_001261276.1 NZ_CP047708:c73093-72863 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT147382 NZ_CP062938:1360326-1360634 [Bifidobacterium eulemuris]
ATGACCTACACCATCGAGGATTTCGCTCGAGACCACAACACCGACCCCACTAAAGTAGAGGCCGCAAAAGCACGGATGCT
TGAAGAGATGCGCATATACGAACTCAAAGAGGCTCGCAAAGCGCAGGCGCTGACGCAGAAGCAGCTCGCAAAGCAGATGG
GCGTGAGCCAGAAGCGCGTCAGCATCATCGAATCCGGTGATGTGGATCACGTTATGGTCAGCACACTGAAGAAGTATCTC
AACGGCATCGGCGGTTCACTGAGCATCACCGCCAAACTTCCCAGCGGCGAAACCCTAGAATTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023