Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5077879..5078504 Replicon chromosome
Accession NZ_CP047707
Organism Klebsiella pneumoniae strain Kpn-13

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GVI78_RS24805 Protein ID WP_002882817.1
Coordinates 5077879..5078262 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GVI78_RS24810 Protein ID WP_004150355.1
Coordinates 5078262..5078504 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI78_RS24790 5075245..5076147 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GVI78_RS24795 5076144..5076779 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GVI78_RS24800 5076776..5077705 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GVI78_RS24805 5077879..5078262 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI78_RS24810 5078262..5078504 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GVI78_RS24815 5078709..5079626 + 918 WP_023302328.1 alpha/beta hydrolase -
GVI78_RS24820 5079640..5080581 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GVI78_RS24825 5080626..5081063 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GVI78_RS24830 5081060..5081920 - 861 WP_004146232.1 virulence factor BrkB family protein -
GVI78_RS24835 5081914..5082513 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T147381 WP_002882817.1 NZ_CP047707:c5078262-5077879 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T147381 NZ_CP062938:131212-131538 [Bifidobacterium eulemuris]
ATGATGACTGATGTTCGCCCTTGGCAGGTCCGCAGAGTCTGGTTCCCGTACCGTGAAGATCCCAGCAGAGGCAAGAATCG
ACGCGTCGTAGTCAAGGAAGTCACTGATGATAACTGTGTGGTGATTTACGTGACCAGCAAGATTGAAAAGAGTCACTTTC
CGGATTTCATGTTGCTGAAGGACTGGAATGAAGAGGGATTCGACAGAGCATCCGCAGTGAGATGGAATCGTCTGTTGAAA
GTCCCTATCAGCGCATTTGGAGAACATCTCGGTGATCTGTCTCCGTATGATCGCTTGGAATTGCAGATGCGATACCAGCT
CCGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT147381 WP_004150355.1 NZ_CP047707:c5078504-5078262 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT147381 NZ_CP062938:130665-131222 [Bifidobacterium eulemuris]
ATGGATGTCATGGATGTGGCGGATTTCTTCATCGCAACGGCGAATGAGGGTAACCCCGAAGAAGATGACGGCATGACGAA
CATGAAGCTCAACAAGTTGTTGTTCTTCGCGCAGGCCGCCAGCTTGCAGCGCTTTGGAAAGCCATTGTTCGACGCGCCTT
TGGAGGCGCGGAAGTATGGCCCTGTGGTCAAAGATGTATATCGGACGTTCAGTGGGTATCAGCGTGATGCCATCGCCAAA
GTCGCGCGTCCTTTTGACTGGAAAACCTTGGATCCGGAAGTTCTACAGTTGCTGTGCGACGTGTATCGCACTTACGCGAG
GGATTATTCGGCTATCGGTTTGATGCGGATGACGCATAAGCCGGGTACGCCGTGGAGTCAAGCGTACGAAGAACACAAGA
ATAATGTCATTTCATGCGAGTCCATTCGTGAGTGGGTCAATCAGACACCATTGATGATCGACGAAACGTCAGTGTCGGAT
TCTTTGGTTACCGTGGCGGAGCTAGACCAGCATGGGCACCCGTTCCTTCCAGAGGGATGGGAAGACGATGATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References