Detailed information of TA system TA147376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2530625..2532213
• Replicon: chromosome
• Accession: NZ_CP047707
• Organism: Klebsiella pneumoniae strain Kpn-13

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: GVI78_RS12420
• Protein ID: WP_023301940.1
• Coordinates: 2530625..2531947 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GVI78_RS12425
• Protein ID: WP_024622638.1
• Coordinates: 2531947..2532213 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI78_RS12405	2525681..2528182	+	2502	WP_023342811.1	fimbrial biogenesis outer membrane usher protein	-
GVI78_RS12410	2528170..2529174	+	1005	WP_023301939.1	type 1 fimbrial protein	-
GVI78_RS12415	2529316..2530608	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
GVI78_RS12420	2530625..2531947	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
GVI78_RS12425	2531947..2532213	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GVI78_RS12430	2532478..2532804	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
GVI78_RS12435	2532801..2533301	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GVI78_RS12440	2533279..2534658	-	1380	WP_023301941.1	purine permease	-
GVI78_RS12445	2535004..2536158	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
GVI78_RS12450	2536139..2537062	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T147376 WP_023301940.1 NZ_CP047707:c2531947-2530625 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T147376 NZ_CP062919:c3282752-3282363 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTACTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT147376 WP_024622638.1 NZ_CP047707:c2532213-2531947 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT147376 NZ_CP062919:c3283004-3282759 [Acinetobacter baumannii]
ATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTTTGACCCTTATGT
GAAAAATTATTATTTAAAGGCAGAGCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAGACCCTGATTTAC
TCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAACACTATGTTCAT
GGTTAA

Structures

Toxin

• AlphaFold DB: A0A486S9S0