Detailed information of TA system TA147364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 239750..240420
• Replicon: plasmid pKpn14-1
• Accession: NZ_CP047701
• Organism: Klebsiella pneumoniae strain Kpn-14

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: GVI79_RS27725
• Protein ID: WP_004213072.1
• Coordinates: 239750..240193 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: GVI79_RS27730
• Protein ID: WP_004213073.1
• Coordinates: 240190..240420 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI79_RS27690	235155..235430	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
GVI79_RS27695	235493..235984	+	492	WP_011251327.1	DM13 domain-containing protein	-
GVI79_RS27700	236033..236953	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
GVI79_RS27705	237044..237450	+	407	Protein_248	GAF domain-containing protein	-
GVI79_RS27710	237968..238606	-	639	Protein_249	response regulator	-
GVI79_RS27715	239023..239316	+	294	Protein_250	transposase	-
GVI79_RS27720	239320..239736	+	417	Protein_251	restriction endonuclease	-
GVI79_RS27725	239750..240193	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI79_RS27730	240190..240420	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GVI79_RS27735	241028..242161	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
GVI79_RS27740	242177..242470	+	294	WP_004213076.1	hypothetical protein	-
GVI79_RS27745	242460..242666	-	207	WP_004213077.1	hypothetical protein	-
GVI79_RS27750	243018..243308	+	291	WP_004213078.1	hypothetical protein	-
GVI79_RS27755	243298..244197	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-1 / tet(B) / sul1 / qacE / ant(3'')-Ia / aph(3')-Ia / aac(3)-IIa / blaTEM-1B	iucA / iucB / iucC / iucD / iutA / rmpA2	1..279435	279435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T147364 WP_004213072.1 NZ_CP047701:c240193-239750 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T147364 NZ_CP062915:c52487-52206 [Escherichia coli O112ab:H8]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT147364 WP_004213073.1 NZ_CP047701:c240420-240190 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT147364 NZ_CP062915:c52753-52484 [Escherichia coli O112ab:H8]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620