Detailed information of TA system TA147362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5280558..5281183
• Replicon: chromosome
• Accession: NZ_CP047700
• Organism: Klebsiella pneumoniae strain Kpn-14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GVI79_RS26100
• Protein ID: WP_062921072.1
• Coordinates: 5280558..5280941 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: GVI79_RS26105
• Protein ID: WP_004150355.1
• Coordinates: 5280941..5281183 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI79_RS26085	5277924..5278826	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
GVI79_RS26090	5278823..5279458	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
GVI79_RS26095	5279455..5280384	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
GVI79_RS26100	5280558..5280941	-	384	WP_062921072.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI79_RS26105	5280941..5281183	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
GVI79_RS26110	5281388..5282305	+	918	WP_062921073.1	alpha/beta hydrolase	-
GVI79_RS26115	5282319..5283260	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
GVI79_RS26120	5283305..5283742	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GVI79_RS26125	5283739..5284599	-	861	WP_004146232.1	virulence factor BrkB family protein	-
GVI79_RS26130	5284593..5285192	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T147362 WP_062921072.1 NZ_CP047700:c5280941-5280558 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDALILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T147362 NZ_CP062914:34367-34888 [Escherichia coli O112ab:H8]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT147362 WP_004150355.1 NZ_CP047700:c5281183-5280941 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT147362 NZ_CP062914:34090-34359 [Escherichia coli O112ab:H8]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9