Detailed information of TA system TA147345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 200604..201240
• Replicon: chromosome
• Accession: NZ_CP047699
• Organism: Priestia megaterium strain 5-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: GVI46_RS01135
• Protein ID: WP_013055004.1
• Coordinates: 200890..201240 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: GVI46_RS01130
• Protein ID: WP_013055003.1
• Coordinates: 200604..200885 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI46_RS01105	195970..196941	+	972	WP_035439664.1	UV DNA damage repair endonuclease UvsE	-
GVI46_RS01110	196950..197552	-	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
GVI46_RS01115	197617..197982	+	366	WP_013055000.1	holo-ACP synthase	-
GVI46_RS01120	198041..199099	+	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
GVI46_RS01125	199213..200403	+	1191	WP_013055002.1	alanine racemase	-
GVI46_RS01130	200604..200885	+	282	WP_013055003.1	hypothetical protein	Antitoxin
GVI46_RS01135	200890..201240	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GVI46_RS01140	201398..202234	+	837	WP_013055005.1	STAS domain-containing protein	-
GVI46_RS01145	202237..202593	+	357	WP_013055006.1	STAS domain-containing protein	-
GVI46_RS01150	202597..202998	+	402	WP_013055007.1	anti-sigma regulatory factor	-
GVI46_RS01155	203012..204022	+	1011	WP_098240004.1	PP2C family protein-serine/threonine phosphatase	-
GVI46_RS01160	204082..204414	+	333	WP_013055009.1	anti-sigma factor antagonist	-
GVI46_RS01165	204411..204896	+	486	WP_013055010.1	anti-sigma B factor RsbW	-
GVI46_RS01170	204862..205656	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T147345 WP_013055004.1 NZ_CP047699:200890-201240 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T147345 NZ_CP062910:341859-341962 [Escherichia coli O112ab:H8]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT147345 WP_013055003.1 NZ_CP047699:200604-200885 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT147345 NZ_CP062910:c342000-341729 [Escherichia coli O112ab:H8]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9