Detailed information of TA system TA147344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4323887..4325026
• Replicon: chromosome
• Accession: NZ_CP047698
• Organism: Pseudomonas knackmussii strain N1-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GVI47_RS20310
• Protein ID: WP_160287936.1
• Coordinates: 4323887..4324657 (-)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A024HLU8
• Locus tag: GVI47_RS20315
• Protein ID: WP_043254039.1
• Coordinates: 4324658..4325026 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI47_RS20290	4319279..4320166	+	888	WP_043254027.1	metal-dependent hydrolase	-
GVI47_RS20295	4320250..4321593	+	1344	WP_160287934.1	FAD-binding oxidoreductase	-
GVI47_RS20300	4321710..4323071	-	1362	WP_156958146.1	DNA recombination protein RmuC	-
GVI47_RS20305	4323600..4323812	-	213	WP_160287935.1	hypothetical protein	-
GVI47_RS20310	4323887..4324657	-	771	WP_160287936.1	RES family NAD+ phosphorylase	Toxin
GVI47_RS20315	4324658..4325026	-	369	WP_043254039.1	XRE family transcriptional regulator	Antitoxin
GVI47_RS20320	4325245..4325649	+	405	WP_160287937.1	RcnB family protein	-
GVI47_RS20325	4325662..4326084	-	423	WP_160287938.1	hypothetical protein	-
GVI47_RS20330	4326215..4326685	+	471	WP_160287939.1	GreA/GreB family elongation factor	-
GVI47_RS20335	4326755..4327096	+	342	WP_043254049.1	carboxymuconolactone decarboxylase family protein	-
GVI47_RS20340	4327152..4327523	+	372	WP_043254051.1	hypothetical protein	-
GVI47_RS20345	4327511..4328566	-	1056	WP_160287940.1	diguanylate cyclase	-
GVI47_RS20355	4328896..4329606	-	711	WP_043254055.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (68-215)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 28955.91 Da
• Isoelectric Point: 7.7884

>T147344 WP_160287936.1 NZ_CP047698:c4324657-4323887 [Pseudomonas knackmussii]
MASDAGAELWRRCQGEQTITPLRGKLVRLVESQEQVATLQLVDTLEEQALLEELIETSKPRLPQTTTDLHYLLKTPFRYP
PLRWGSRFGRRHEPSLFYAALRLETAMAEGAYYRFVLWEGMSSPPPSGRILSEHASFEARFQVQRGVRLQQAPFAEHAAR
LEDRQDYGLTQGLGSAMRGAGVEAFEYRSARCPLGGLNAALFAPSAFVDRRPRNLQPWLCETTGDYVAYKQAQVPDAPRL
FPRETFLVDGRLPYPA

Nucleotide

• Length: 771 bp

>T147344 NZ_CP062910:105712-105966 [Escherichia coli O112ab:H8]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13376.56 Da
• Isoelectric Point: 7.3187

>AT147344 WP_043254039.1 NZ_CP047698:c4325026-4324658 [Pseudomonas knackmussii]
MSALIQDSPSPDIVLAKAVLAAREQLGMTQQELADIVGVDRSAVSRWKGAGLRVNSKTGELALLLVRIYRALFALFGGNL
EDMRHFLRTDNHHLGGVPLQLMEQVQGLVQVVEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT147344 NZ_CP062910:105464-105715 [Escherichia coli O112ab:H8]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A024HLU8