Detailed information of TA system TA147343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 4308877..4310312
• Replicon: chromosome
• Accession: NZ_CP047698
• Organism: Pseudomonas knackmussii strain N1-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GVI47_RS20255
• Protein ID: WP_160287930.1
• Coordinates: 4309749..4310312 (-)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GVI47_RS20250
• Protein ID: WP_201752598.1
• Coordinates: 4308877..4309725 (-)
• Length: 283 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI47_RS20225	4304082..4305002	+	921	WP_043253999.1	recombination-associated protein RdgC	-
GVI47_RS20230	4305073..4306011	+	939	WP_160287927.1	bile acid:sodium symporter family protein	-
GVI47_RS20235	4306145..4306459	+	315	WP_043254003.1	quaternary ammonium compound efflux SMR transporter SugE	-
GVI47_RS20240	4306619..4306828	+	210	WP_043254005.1	cold-shock protein	-
GVI47_RS20245	4306877..4308751	-	1875	WP_160287928.1	MFS transporter	-
GVI47_RS20250	4308877..4309725	-	849	WP_201752598.1	AraC family transcriptional regulator	Antitoxin
GVI47_RS20255	4309749..4310312	-	564	WP_160287930.1	GNAT family N-acetyltransferase	Toxin
GVI47_RS20260	4310565..4314044	+	3480	WP_160287931.1	hybrid sensor histidine kinase/response regulator	-
GVI47_RS20265	4314056..4314514	-	459	WP_043254014.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-144)

Antitoxin

• DNA binding HTH domain, AraC-type (196-272)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 20884.08 Da
• Isoelectric Point: 9.9885

>T147343 WP_160287930.1 NZ_CP047698:c4310312-4309749 [Pseudomonas knackmussii]
MFRPSPITLQRGALRLEPMVEADVSELVALAEANREALQFMSAPQRPDWYRQSIADQREGRALPLVVRLQIVGTTRFMDF
LPALPACEIGGTWLDAAQHGSGLNASIKYLMLRQAFDNWKMVRVQLKTAASNLRSQRAIEKLGAVREGVLRNHRRLAGGR
LDDSVIYSITDLEWPQVKAALEAGFNG

Nucleotide

• Length: 564 bp

>T147343 NZ_CP062910:105464-105715 [Escherichia coli O112ab:H8]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 283 a.a.
• Molecular weight: 31351.60 Da
• Isoelectric Point: 8.5430

>AT147343 WP_201752598.1 NZ_CP047698:c4309725-4308877 [Pseudomonas knackmussii]
MQDDAQGERTRFWRAQELGGLELLHARYIGQRFAPHVHEGFSLVLIEQGAQRFRHRGAEHLAPSGSLVLINPDEVHTGSK
AHDAGWQYRGFYPEIDQVGSVLDELSLGGRGLPTFDASVLHDPQLSRAFIHVHRLLESPASDLERQTCWREALLALFQRH
ARVPEARPFGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHLSRVFRQATGLPPHAWIKQRRLEQARALLKQGGA
PAMVAAQLGFADQSHLSRQFKQAYGVAPGEYRLACGRSIISS

Nucleotide

• Length: 849 bp

>AT147343 NZ_CP062910:105712-105966 [Escherichia coli O112ab:H8]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA