Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-StbC
Location 854782..855443 Replicon chromosome
Accession NZ_CP047698
Organism Pseudomonas knackmussii strain N1-2

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag GVI47_RS04035 Protein ID WP_160285818.1
Coordinates 855024..855443 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag GVI47_RS04030 Protein ID WP_160288868.1
Coordinates 854782..855027 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI47_RS04005 850313..851176 + 864 WP_160285813.1 restriction endonuclease -
GVI47_RS04010 851357..851911 + 555 WP_160285814.1 hypothetical protein -
GVI47_RS04015 851911..852927 + 1017 WP_160285815.1 DUF262 domain-containing protein -
GVI47_RS04020 853727..853999 + 273 WP_160285816.1 DNA-binding protein -
GVI47_RS04025 854171..854671 + 501 WP_160285817.1 hypothetical protein -
GVI47_RS04030 854782..855027 + 246 WP_160288868.1 Arc family DNA-binding protein Antitoxin
GVI47_RS04035 855024..855443 + 420 WP_160285818.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI47_RS04040 855478..855654 + 177 WP_160285819.1 hypothetical protein -
GVI47_RS04050 855984..856886 - 903 WP_160285820.1 LysR family transcriptional regulator -
GVI47_RS04055 857174..858679 + 1506 WP_160285821.1 cytosine permease -
GVI47_RS04060 858703..859548 + 846 WP_160285822.1 nitrilase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(11-43)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15259.40 Da        Isoelectric Point: 4.6331

>T147342 WP_160285818.1 NZ_CP047698:855024-855443 [Pseudomonas knackmussii]
MILLDTNVLSELMRAKPDPAVVAWIDAQPADDLHISAITVAEILYGIARMPDGKRKQALHDLALVMFEEDFAGNILPFDT
DAAVHYAELVAESESRGKIADMADGQIAAIARLHGAQIATRNVRHFEHFGVSLTDPWKD

Download         Length: 420 bp

>T147342 NZ_CP062909:c28837-28421 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8791.04 Da        Isoelectric Point: 10.2879

>AT147342 WP_160288868.1 NZ_CP047698:854782-855027 [Pseudomonas knackmussii]
MASITIRNLDEDLKAKLRVVAASHGRSMEEEVRVILAQALSRDVKRGGLGSRIHARFASMGGADIEIPARTSKARAADFD
E

Download         Length: 246 bp

>AT147342 NZ_CP062909:c29064-28834 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References