Detailed information of TA system TA147342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 854782..855443
• Replicon: chromosome
• Accession: NZ_CP047698
• Organism: Pseudomonas knackmussii strain N1-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GVI47_RS04035
• Protein ID: WP_160285818.1
• Coordinates: 855024..855443 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GVI47_RS04030
• Protein ID: WP_160288868.1
• Coordinates: 854782..855027 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI47_RS04005	850313..851176	+	864	WP_160285813.1	restriction endonuclease	-
GVI47_RS04010	851357..851911	+	555	WP_160285814.1	hypothetical protein	-
GVI47_RS04015	851911..852927	+	1017	WP_160285815.1	DUF262 domain-containing protein	-
GVI47_RS04020	853727..853999	+	273	WP_160285816.1	DNA-binding protein	-
GVI47_RS04025	854171..854671	+	501	WP_160285817.1	hypothetical protein	-
GVI47_RS04030	854782..855027	+	246	WP_160288868.1	Arc family DNA-binding protein	Antitoxin
GVI47_RS04035	855024..855443	+	420	WP_160285818.1	type II toxin-antitoxin system VapC family toxin	Toxin
GVI47_RS04040	855478..855654	+	177	WP_160285819.1	hypothetical protein	-
GVI47_RS04050	855984..856886	-	903	WP_160285820.1	LysR family transcriptional regulator	-
GVI47_RS04055	857174..858679	+	1506	WP_160285821.1	cytosine permease	-
GVI47_RS04060	858703..859548	+	846	WP_160285822.1	nitrilase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (11-43)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15259.40 Da
• Isoelectric Point: 4.6331

>T147342 WP_160285818.1 NZ_CP047698:855024-855443 [Pseudomonas knackmussii]
MILLDTNVLSELMRAKPDPAVVAWIDAQPADDLHISAITVAEILYGIARMPDGKRKQALHDLALVMFEEDFAGNILPFDT
DAAVHYAELVAESESRGKIADMADGQIAAIARLHGAQIATRNVRHFEHFGVSLTDPWKD

Nucleotide

• Length: 420 bp

>T147342 NZ_CP062909:c28837-28421 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8791.04 Da
• Isoelectric Point: 10.2879

>AT147342 WP_160288868.1 NZ_CP047698:854782-855027 [Pseudomonas knackmussii]
MASITIRNLDEDLKAKLRVVAASHGRSMEEEVRVILAQALSRDVKRGGLGSRIHARFASMGGADIEIPARTSKARAADFD
E

Nucleotide

• Length: 246 bp

>AT147342 NZ_CP062909:c29064-28834 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA