Detailed information of TA system TA147334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 4314312..4314822
• Replicon: chromosome
• Accession: NZ_CP047696
• Organism: Marinomonas mediterranea MMB-1

Toxin (Protein)

• Gene name: relB
• Uniprot ID: F2JYH1
• Locus tag: GV053_RS19785
• Protein ID: WP_013663002.1
• Coordinates: 4314312..4314575 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: F2JYH2
• Locus tag: GV053_RS19790
• Protein ID: WP_013663003.1
• Coordinates: 4314568..4314822 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV053_RS19755 (GV053_19675)	4309379..4309581	-	203	Protein_3840	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GV053_RS19760 (GV053_19680)	4309884..4311431	+	1548	WP_013662997.1	hypothetical protein	-
GV053_RS19765 (GV053_19685)	4311441..4311914	-	474	WP_013662998.1	hypothetical protein	-
GV053_RS19770 (GV053_19690)	4312095..4313261	+	1167	WP_013662999.1	cell division protein ZapE	-
GV053_RS19775 (GV053_19695)	4313445..4313888	-	444	WP_013663000.1	GNAT family N-acetyltransferase	-
GV053_RS19780 (GV053_19700)	4313952..4314206	-	255	WP_223294992.1	hypothetical protein	-
GV053_RS19785 (GV053_19705)	4314312..4314575	-	264	WP_013663002.1	Txe/YoeB family addiction module toxin	Toxin
GV053_RS19790 (GV053_19710)	4314568..4314822	-	255	WP_013663003.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GV053_RS19795 (GV053_19715)	4314924..4315445	-	522	WP_013663004.1	DUF2569 domain-containing protein	-
GV053_RS19800 (GV053_19720)	4315962..4317443	-	1482	WP_013663005.1	carboxypeptidase M32	-
GV053_RS19805 (GV053_19725)	4317733..4318599	+	867	WP_013663006.1	DMT family transporter	-
GV053_RS19810 (GV053_19730)	4318684..4319133	+	450	WP_013663007.1	hypothetical protein	-
GV053_RS19815 (GV053_19735)	4319121..4319651	+	531	WP_013663008.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10256.69 Da
• Isoelectric Point: 9.4082

>T147334 WP_013663002.1 NZ_CP047696:c4314575-4314312 [Marinomonas mediterranea MMB-1]
MSDRLLSWTDEAWNGYVYWQTQDKKTLKRINKLIADVKRSPFVGIGKPESLKENLSGFWSRRIDDTNRLVYAVDDIAITV
ISCRYHY

Nucleotide

• Length: 264 bp

>T147334 NZ_CP062908:c2434326-2434150 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.67 Da
• Isoelectric Point: 5.0623

>AT147334 WP_013663003.1 NZ_CP047696:c4314822-4314568 [Marinomonas mediterranea MMB-1]
MRIVSFTEARNGLKAVLDNVINDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKTTQRE
LIDE

Nucleotide

• Length: 255 bp

>AT147334 NZ_CP062908:c2434104-2433688 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: F2JYH1

Antitoxin

• AlphaFold DB: F2JYH2