Detailed information of TA system TA147329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4078177..4078899
• Replicon: chromosome
• Accession: NZ_CP047695
• Organism: Marinomonas mediterranea strain MMB-2 (IVIA-Po-186)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GV054_RS18730
• Protein ID: WP_272641689.1
• Coordinates: 4078717..4078899 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: F2JW02
• Locus tag: GV054_RS18725
• Protein ID: WP_013662792.1
• Coordinates: 4078177..4078581 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV054_RS18710 (GV054_18625)	4075083..4075856	-	774	WP_013662789.1	DotU family type IV/VI secretion system protein	-
GV054_RS18715 (GV054_18630)	4075861..4077198	-	1338	WP_013662790.1	type VI secretion system baseplate subunit TssK	-
GV054_RS18720 (GV054_18635)	4077320..4077778	-	459	WP_013662791.1	type VI secretion system lipoprotein TssJ	-
GV054_RS18725 (GV054_18640)	4078177..4078581	-	405	WP_013662792.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GV054_RS18730 (GV054_18645)	4078717..4078899	-	183	WP_272641689.1	type II toxin-antitoxin system HicA family toxin	Toxin
GV054_RS18745 (GV054_18660)	4079798..4080271	+	474	WP_013662805.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
GV054_RS18750 (GV054_18665)	4080280..4081380	+	1101	WP_272643489.1	potassium channel protein	-
GV054_RS18755 (GV054_18670)	4081473..4082012	-	540	WP_013662807.1	DUF924 family protein	-
GV054_RS18760 (GV054_18675)	4082133..4082384	-	252	WP_013662808.1	YfhL family 4Fe-4S dicluster ferredoxin	-
GV054_RS18765 (GV054_18680)	4082444..4082932	-	489	WP_013662809.1	pantetheine-phosphate adenylyltransferase	-
GV054_RS18770 (GV054_18685)	4083089..4083631	-	543	WP_272643490.1	YggT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6873.96 Da
• Isoelectric Point: 10.6196

>T147329 WP_272641689.1 NZ_CP047695:c4078899-4078717 [Marinomonas mediterranea]
MRSREVIKELERDGWYEVATKGSHVQFKHPTKKGRVTVPHPKSDISIGTLKSIMKQAQLD

Nucleotide

• Length: 183 bp

>T147329 NZ_CP062908:1797227-1797478 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14551.53 Da
• Isoelectric Point: 4.3042

>AT147329 WP_013662792.1 NZ_CP047695:c4078581-4078177 [Marinomonas mediterranea]
MKYPIVLHTDDGDSYSVTVPDVPGCFSAGTTLNEAIDMAEEAIFFHLEGMLEDGEDVPVASEIAVYKDNPDFADGIWAVA
SVDITPLLGKSEKINVTLPHLLISKIDKAVASNPQYKNRSAFLAQVSRKELKLA

Nucleotide

• Length: 405 bp

>AT147329 NZ_CP062908:1797475-1797729 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: F2JW02