Detailed information of TA system TA147327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4508656..4510363
• Replicon: chromosome
• Accession: NZ_CP047694
• Organism: Marinomonas mediterranea strain MMB-3 (CPR1)

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GV055_RS20645
• Protein ID: WP_272641883.1
• Coordinates: 4508656..4509579 (-)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: GV055_RS20650
• Protein ID: WP_272641884.1
• Coordinates: 4509572..4510363 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV055_RS20635 (GV055_20580)	4505520..4506689	-	1170	WP_272641881.1	DNA cytosine methyltransferase	-
GV055_RS20640 (GV055_20590)	4507889..4508629	+	741	WP_272641882.1	class I SAM-dependent methyltransferase	-
GV055_RS20645 (GV055_20595)	4508656..4509579	-	924	WP_272641883.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GV055_RS20650 (GV055_20600)	4509572..4510363	-	792	WP_272641884.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GV055_RS20655 (GV055_20605)	4510576..4512348	-	1773	WP_272641885.1	diguanylate cyclase	-
GV055_RS20660 (GV055_20610)	4512527..4513249	-	723	WP_041649062.1	RNA methyltransferase	-
GV055_RS20665 (GV055_20615)	4513438..4514058	+	621	WP_013663175.1	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	-
GV055_RS20670 (GV055_20620)	4514163..4515230	+	1068	WP_013663176.1	23S rRNA pseudouridine(2604) synthase RluF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4483093..4675957	192864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-279)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-96)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 35050.45 Da
• Isoelectric Point: 5.0239

>T147327 WP_272641883.1 NZ_CP047694:c4509579-4508656 [Marinomonas mediterranea]
MDRESPYYKQLSLLIRMLPIVATESVFALKGGTAINLFVRNFPRLSVDIDLAYLPLEPRIEALENVKAALKRIVDLINAQ
PDTRAVLQDQKVDELRIVVTSLNATIKIEVSPVARGTLHKPNNLPVQELVEEEYGYAEISVVSLPDLYGGKLCAAMDRQH
PRDLFDVRTLLLEEGISRDIFIGFITYALSHPRPINEVMAPNWQPLDETFKAEFDGMTFEPVNLEDLLSVRSKMVNELKK
HFTKEDRAFLLSFKQGNPDWTLFDYPDAANLPAIRWKLQNLDKLAKNQAKHKEQLAKLEAVLDEWLE

Nucleotide

• Length: 924 bp

>T147327 NZ_CP062908:867792-868199 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 29915.38 Da
• Isoelectric Point: 9.6898

>AT147327 WP_272641884.1 NZ_CP047694:c4510363-4509572 [Marinomonas mediterranea]
MSSKINWLVTHTSPGALVLQQWLTENGISYSLAQKYTKSGWLMKLSSGVYYRPSANGDSKPDWTEALQATDIQLRLPVHL
AGLGSLTHQGLSHYLSVGKEQVWVGVKSKQSLPKWFREFPDQNWFYCGNHKLEIVPEKDLKVIMVKGKALKASCPELAAY
EVVDAIGKLISFEHAAELFQGLVNLSPRKVQSLLERSRSVQTNRVFLFLCHFYDHQWVQRLDETKIGLGSGKRQVVEKGR
LDERYQITVPEKFLAKQEEQKNG

Nucleotide

• Length: 792 bp

>AT147327 NZ_CP062908:867545-867811 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA