Detailed information of TA system TA147324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 4302743..4303253
• Replicon: chromosome
• Accession: NZ_CP047694
• Organism: Marinomonas mediterranea strain MMB-3 (CPR1)

Toxin (Protein)

• Gene name: relB
• Uniprot ID: F2JYH1
• Locus tag: GV055_RS19785
• Protein ID: WP_013663002.1
• Coordinates: 4302743..4303006 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: F2JYH2
• Locus tag: GV055_RS19790
• Protein ID: WP_013663003.1
• Coordinates: 4302999..4303253 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV055_RS19760 (GV055_19705)	4299422..4299649	+	228	WP_272641785.1	DUF2798 domain-containing protein	-
GV055_RS19765 (GV055_19710)	4299813..4300262	-	450	WP_272641786.1	pyridoxamine 5'-phosphate oxidase family protein	-
GV055_RS19770 (GV055_19715)	4300525..4301691	+	1167	WP_272641787.1	cell division protein ZapE	-
GV055_RS19775 (GV055_19720)	4301876..4302319	-	444	WP_272641788.1	GNAT family N-acetyltransferase	-
GV055_RS19780 (GV055_19725)	4302383..4302637	-	255	WP_223294992.1	hypothetical protein	-
GV055_RS19785 (GV055_19730)	4302743..4303006	-	264	WP_013663002.1	Txe/YoeB family addiction module toxin	Toxin
GV055_RS19790 (GV055_19735)	4302999..4303253	-	255	WP_013663003.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GV055_RS19795 (GV055_19740)	4303599..4303949	-	351	WP_272641789.1	hypothetical protein	-
GV055_RS19800 (GV055_19745)	4304059..4304406	-	348	WP_272641790.1	hypothetical protein	-
GV055_RS19805 (GV055_19750)	4304477..4304965	-	489	WP_013795848.1	DUF695 domain-containing protein	-
GV055_RS19810 (GV055_19755)	4305658..4306845	-	1188	WP_272641791.1	threonine--tRNA ligase	-
GV055_RS19815 (GV055_19760)	4307251..4307595	-	345	WP_272641792.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10256.69 Da
• Isoelectric Point: 9.4082

>T147324 WP_013663002.1 NZ_CP047694:c4303006-4302743 [Marinomonas mediterranea]
MSDRLLSWTDEAWNGYVYWQTQDKKTLKRINKLIADVKRSPFVGIGKPESLKENLSGFWSRRIDDTNRLVYAVDDIAITV
ISCRYHY

Nucleotide

• Length: 264 bp

>T147324 NZ_CP062908:662395-662709 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.67 Da
• Isoelectric Point: 5.0623

>AT147324 WP_013663003.1 NZ_CP047694:c4303253-4302999 [Marinomonas mediterranea]
MRIVSFTEARNGLKAVLDNVINDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKTTQRE
LIDE

Nucleotide

• Length: 255 bp

>AT147324 NZ_CP062908:662706-663122 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: F2JYH1

Antitoxin

• AlphaFold DB: F2JYH2