Detailed information of TA system TA147323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4095702..4096379
• Replicon: chromosome
• Accession: NZ_CP047694
• Organism: Marinomonas mediterranea strain MMB-3 (CPR1)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GV055_RS18820
• Protein ID: WP_272641689.1
• Coordinates: 4096197..4096379 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: F2JW02
• Locus tag: GV055_RS18815
• Protein ID: WP_013662792.1
• Coordinates: 4095702..4096106 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV055_RS18800 (GV055_18745)	4092608..4093381	-	774	WP_013662789.1	DotU family type IV/VI secretion system protein	-
GV055_RS18805 (GV055_18750)	4093386..4094723	-	1338	WP_272641688.1	type VI secretion system baseplate subunit TssK	-
GV055_RS18810 (GV055_18755)	4094845..4095303	-	459	WP_013662791.1	type VI secretion system lipoprotein TssJ	-
GV055_RS18815 (GV055_18760)	4095702..4096106	-	405	WP_013662792.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GV055_RS18820 (GV055_18765)	4096197..4096379	-	183	WP_272641689.1	type II toxin-antitoxin system HicA family toxin	Toxin
GV055_RS18835 (GV055_18780)	4097277..4097750	+	474	WP_013662805.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
GV055_RS18840 (GV055_18785)	4097759..4098859	+	1101	WP_272641690.1	potassium channel protein	-
GV055_RS18845 (GV055_18790)	4098952..4099491	-	540	WP_013662807.1	DUF924 family protein	-
GV055_RS18850 (GV055_18795)	4099612..4099863	-	252	WP_272641691.1	YfhL family 4Fe-4S dicluster ferredoxin	-
GV055_RS18855 (GV055_18800)	4099923..4100411	-	489	WP_013662809.1	pantetheine-phosphate adenylyltransferase	-
GV055_RS18860 (GV055_18805)	4100568..4101110	-	543	WP_272641692.1	YggT family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6873.96 Da
• Isoelectric Point: 10.6196

>T147323 WP_272641689.1 NZ_CP047694:c4096379-4096197 [Marinomonas mediterranea]
MRSREVIKELERDGWYEVATKGSHVQFKHPTKKGRVTVPHPKSDISIGTLKSIMKQAQLD

Nucleotide

• Length: 183 bp

>T147323 NZ_CP062908:c616975-616511 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14551.53 Da
• Isoelectric Point: 4.3042

>AT147323 WP_013662792.1 NZ_CP047694:c4096106-4095702 [Marinomonas mediterranea]
MKYPIVLHTDDGDSYSVTVPDVPGCFSAGTTLNEAIDMAEEAIFFHLEGMLEDGEDVPVASEIAVYKDNPDFADGIWAVA
SVDITPLLGKSEKINVTLPHLLISKIDKAVASNPQYKNRSAFLAQVSRKELKLA

Nucleotide

• Length: 405 bp

>AT147323 NZ_CP062908:c617310-616975 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: F2JW02