Detailed information of TA system TA147321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 884551..885572
• Replicon: chromosome
• Accession: NZ_CP047694
• Organism: Marinomonas mediterranea strain MMB-3 (CPR1)

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GV055_RS04175
• Protein ID: WP_272642347.1
• Coordinates: 885003..885572 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: GV055_RS04170
• Protein ID: WP_272642346.1
• Coordinates: 884551..885003 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV055_RS04150 (GV055_04140)	879869..880657	-	789	WP_272642342.1	TIM barrel protein	-
GV055_RS04155 (GV055_04145)	880990..881898	-	909	WP_272642343.1	sugar ABC transporter substrate-binding protein	-
GV055_RS04160 (GV055_04150)	882159..883067	+	909	WP_272642344.1	LacI family DNA-binding transcriptional regulator	-
GV055_RS04165 (GV055_04155)	883188..884348	+	1161	WP_272642345.1	phytanoyl-CoA dioxygenase family protein	-
GV055_RS04170 (GV055_04160)	884551..885003	+	453	WP_272642346.1	helix-turn-helix domain-containing protein	Antitoxin
GV055_RS04175 (GV055_04165)	885003..885572	+	570	WP_272642347.1	PIN domain-containing protein	Toxin
GV055_RS04180 (GV055_04170)	885632..885796	+	165	Protein_811	tyrosine-type recombinase/integrase	-
GV055_RS04185 (GV055_04175)	885930..887027	+	1098	WP_272642348.1	nucleoid-associated protein	-
GV055_RS04190 (GV055_04180)	887020..888372	+	1353	WP_272642349.1	hypothetical protein	-
GV055_RS04200 (GV055_04190)	888819..889487	+	669	WP_013660011.1	glutathione S-transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21356.57 Da
• Isoelectric Point: 6.1361

>T147321 WP_272642347.1 NZ_CP047694:885003-885572 [Marinomonas mediterranea]
MNTYTVILDACVLYPAPLRSYLMYLASTGLFRARWSDQIHEEWIRNLLINRPTINPAALQRSRELMDSHVPDALVQGHEA
LIDGLRLPDMGDRHVLAAAIQGHAEGIITFNLKDFPNENLAPFGISALHPDEFLSDMFELDSAACLLAAQRHRKSLKNPP
FNSDEYLECLLKQKLPSFVSDLRKLSFAL

Nucleotide

• Length: 570 bp

>T147321 NZ_CP062907:62281-62433 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16668.96 Da
• Isoelectric Point: 4.5467

>AT147321 WP_272642346.1 NZ_CP047694:884551-885003 [Marinomonas mediterranea]
MRTTTRTPSQEEAALAKLCSQELAGVLDTNGDEQNFSFTDKSGKTHDIVLPKPALNLFIEVLTQLGEGNIVQITPIHAEL
TTQQAADMLNMSRPSLIKLLDEEVIPFSRTGNRRKVAYVDVMSYKDSLLQKRLASLDELSALDQEIDMGY

Nucleotide

• Length: 453 bp

>AT147321 NZ_CP062907:c62226-62169 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT