Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 5872..6397 | Replicon | plasmid p1140-KPC |
| Accession | NZ_CP047689 | ||
| Organism | Serratia marcescens strain 1140- | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | D4HQE7 |
| Locus tag | GV224_RS25840 | Protein ID | WP_013023785.1 |
| Coordinates | 5872..6177 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | W8V2V6 |
| Locus tag | GV224_RS25845 | Protein ID | WP_001568025.1 |
| Coordinates | 6179..6397 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GV224_RS25815 | 1551..2177 | + | 627 | WP_004197649.1 | ParA family plasmid-partitioning AAA ATPase | - |
| GV224_RS25820 | 2297..2476 | + | 180 | WP_006788217.1 | hypothetical protein | - |
| GV224_RS25825 | 2948..3742 | - | 795 | WP_004197635.1 | site-specific integrase | - |
| GV224_RS25830 | 3940..4956 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| GV224_RS25835 | 5308..5703 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| GV224_RS25840 | 5872..6177 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| GV224_RS25845 | 6179..6397 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| GV224_RS26570 | 6567..7028 | - | 462 | WP_160866775.1 | hypothetical protein | - |
| GV224_RS25850 | 6985..7215 | + | 231 | WP_001261282.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| GV224_RS25855 | 7212..7628 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | - |
| GV224_RS25860 | 7702..9264 | + | 1563 | WP_004206609.1 | AAA family ATPase | - |
| GV224_RS25865 | 9249..10271 | + | 1023 | WP_000361404.1 | helicase UvrD | - |
| GV224_RS25870 | 10527..11224 | + | 698 | Protein_13 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaKPC-2 / qnrS1 | - | 1..103167 | 103167 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11615.30 Da Isoelectric Point: 6.4672
>T147297 WP_013023785.1 NZ_CP047689:c6177-5872 [Serratia marcescens]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T147297 NZ_CP062901:183348-183455 [Escherichia coli O152:H23]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAATAA
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT147297 WP_001568025.1 NZ_CP047689:c6397-6179 [Serratia marcescens]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT147297 NZ_CP062901:c183291-183236 [Escherichia coli O152:H23]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZP8 |