Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 14718..15319 Replicon chromosome
Accession NZ_CP047688
Organism Serratia marcescens strain 1140-

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V4FS51
Locus tag GV224_RS00070 Protein ID WP_033649548.1
Coordinates 14718..15098 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A086G6A0
Locus tag GV224_RS00075 Protein ID WP_004933932.1
Coordinates 15098..15319 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GV224_RS00045 9971..11251 + 1281 WP_047569945.1 DUF3748 domain-containing protein -
GV224_RS00050 11282..11629 - 348 WP_033636538.1 YceK/YidQ family lipoprotein -
GV224_RS00055 11940..12353 + 414 WP_047569943.1 heat shock chaperone IbpA -
GV224_RS00060 12454..12882 + 429 WP_004933922.1 heat shock chaperone IbpB -
GV224_RS00065 13049..14707 + 1659 WP_047569941.1 putative transporter -
GV224_RS00070 14718..15098 - 381 WP_033649548.1 type II toxin-antitoxin system VapC family toxin Toxin
GV224_RS00075 15098..15319 - 222 WP_004933932.1 ribbon-helix-helix domain-containing protein Antitoxin
GV224_RS00080 15394..16644 - 1251 WP_033645138.1 valine--pyruvate transaminase -
GV224_RS00085 16780..18843 - 2064 WP_047569940.1 alpha-amylase -
GV224_RS26440 19040..19192 + 153 WP_019455508.1 hypothetical protein -
GV224_RS00090 19194..20171 - 978 WP_047569939.1 glyoxylate/hydroxypyruvate reductase GhrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-102)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14171.50 Da        Isoelectric Point: 7.3170

>T147277 WP_033649548.1 NZ_CP047688:c15098-14718 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIVWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Download         Length: 381 bp

>T147277 NZ_CP062895:2306421-2306708 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8593.53 Da        Isoelectric Point: 4.4791

>AT147277 WP_004933932.1 NZ_CP047688:c15319-15098 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Download         Length: 222 bp

>AT147277 NZ_CP062895:2306182-2306421 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V4FS51

Antitoxin

Source ID Structure
AlphaFold DB A0A086G6A0

References