Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6985..7628 Replicon plasmid p2838-KPC
Accession NZ_CP047686
Organism Serratia marcescens strain 2838

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag GV198_RS25785 Protein ID WP_001044770.1
Coordinates 7212..7628 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag GV198_RS25780 Protein ID WP_001261282.1
Coordinates 6985..7215 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GV198_RS25750 2297..2476 + 180 WP_006788217.1 hypothetical protein -
GV198_RS25755 2948..3742 - 795 WP_004197635.1 site-specific integrase -
GV198_RS25760 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
GV198_RS25765 5308..5703 - 396 WP_017899885.1 hypothetical protein -
GV198_RS25770 5872..6177 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
GV198_RS25775 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
GV198_RS26500 6567..7028 - 462 WP_160866775.1 hypothetical protein -
GV198_RS25780 6985..7215 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GV198_RS25785 7212..7628 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
GV198_RS25790 7702..9264 + 1563 WP_004206609.1 AAA family ATPase -
GV198_RS25795 9249..10271 + 1023 WP_000361404.1 helicase UvrD -
GV198_RS25800 10527..11224 + 698 Protein_13 IS1 family transposase -
GV198_RS25805 11253..11525 + 273 Protein_14 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / qnrS1 - 1..103167 103167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T147276 WP_001044770.1 NZ_CP047686:7212-7628 [Serratia marcescens]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T147276 NZ_CP062895:2019934-2020036 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT147276 WP_001261282.1 NZ_CP047686:6985-7215 [Serratia marcescens]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT147276 NZ_CP062895:c2020074-2019803 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCACTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References