Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6993..7636 Replicon plasmid p3024-KPC
Accession NZ_CP047683
Organism Serratia marcescens strain 3024

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag GV218_RS25860 Protein ID WP_001044770.1
Coordinates 7220..7636 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag GV218_RS25855 Protein ID WP_001261282.1
Coordinates 6993..7223 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GV218_RS25825 2297..2476 + 180 WP_006788217.1 hypothetical protein -
GV218_RS25830 2956..3750 - 795 WP_004197635.1 site-specific integrase -
GV218_RS25835 3948..4964 - 1017 WP_017899884.1 hypothetical protein -
GV218_RS25840 5316..5711 - 396 WP_017899885.1 hypothetical protein -
GV218_RS25845 5880..6185 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
GV218_RS25850 6187..6405 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
GV218_RS26575 6575..7036 - 462 WP_160866775.1 hypothetical protein -
GV218_RS25855 6993..7223 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GV218_RS25860 7220..7636 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
GV218_RS25865 7710..9272 + 1563 WP_004206609.1 AAA family ATPase -
GV218_RS25870 9257..10279 + 1023 WP_000361404.1 helicase UvrD -
GV218_RS25875 10535..11232 + 698 Protein_13 IS1 family transposase -
GV218_RS25880 11261..11533 + 273 Protein_14 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / qnrS1 - 1..103175 103175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T147254 WP_001044770.1 NZ_CP047683:7220-7636 [Serratia marcescens]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T147254 NZ_CP062889:3819514-3819723 [Escherichia coli O100:H21]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTAGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT147254 WP_001261282.1 NZ_CP047683:6993-7223 [Serratia marcescens]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT147254 NZ_CP062889:c3819522-3819465 [Escherichia coli O100:H21]
GTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References