Detailed information of TA system TA147232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 207211..207881
• Replicon: plasmid unnamed
• Accession: NZ_CP047678
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: GO012_RS26400
• Protein ID: WP_004213072.1
• Coordinates: 207438..207881 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: GO012_RS26395
• Protein ID: WP_004213073.1
• Coordinates: 207211..207441 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO012_RS26370	203434..204333	-	900	WP_004225022.1	nucleotide-binding protein	-
GO012_RS26375	204323..204613	-	291	WP_004213078.1	hypothetical protein	-
GO012_RS26380	204965..205171	+	207	WP_004213077.1	hypothetical protein	-
GO012_RS26385	205161..205454	-	294	WP_004213076.1	hypothetical protein	-
GO012_RS26390	205470..206603	-	1134	WP_141450088.1	DUF3800 domain-containing protein	-
GO012_RS26395	207211..207441	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GO012_RS26400	207438..207881	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
GO012_RS26405	207895..208311	-	417	Protein_220	restriction endonuclease	-
GO012_RS26410	208315..208608	-	294	Protein_221	transposase	-
GO012_RS27080	209025..209662	+	638	Protein_222	response regulator	-
GO012_RS26420	210177..210583	-	407	Protein_223	GAF domain-containing protein	-
GO012_RS26425	210674..211594	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
GO012_RS26430	211643..212134	-	492	WP_160188106.1	DM13 domain-containing protein	-
GO012_RS26435	212197..212472	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / rmpA / iutA / iucD / iucC / iucB / iucA	1..229024	229024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T147232 WP_004213072.1 NZ_CP047678:207438-207881 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T147232 NZ_CP062888:c78738-78532 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATACCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT147232 WP_004213073.1 NZ_CP047678:207211-207441 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT147232 NZ_CP062888:78725-78786 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620