Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 30593..31503 Replicon plasmid unnamed
Accession NZ_CP047678
Organism Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag GO012_RS25485 Protein ID WP_004026354.1
Coordinates 31033..31503 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag GO012_RS25480 Protein ID WP_004026357.1
Coordinates 30593..31036 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO012_RS25450 25611..26132 + 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -
GO012_RS25455 26129..27082 + 954 Protein_28 fec operon regulator FecR -
GO012_RS25460 27169..29295 + 2127 WP_004213640.1 TonB-dependent receptor family protein -
GO012_RS25465 29370..29525 + 156 WP_004213642.1 hypothetical protein -
GO012_RS25470 29670..29891 + 222 WP_004213643.1 DUF2188 domain-containing protein -
GO012_RS25475 29989..30492 + 504 Protein_32 DUF4113 domain-containing protein -
GO012_RS25480 30593..31036 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
GO012_RS25485 31033..31503 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
GO012_RS25490 31652..32349 + 698 Protein_35 IS1 family transposase -
GO012_RS25495 33735..34684 - 950 Protein_36 DUF1173 family protein -
GO012_RS25500 34862..35356 + 495 WP_004212794.1 thermonuclease family protein -
GO012_RS25505 35693..36091 + 399 WP_004212796.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / rmpA / iutA / iucD / iucC / iucB / iucA 1..229024 229024
- flank IS/Tn - - 31846..32349 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T147230 WP_004026354.1 NZ_CP047678:31033-31503 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T147230 NZ_CP062887:65159-65311 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT147230 WP_004026357.1 NZ_CP047678:30593-31036 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT147230 NZ_CP062887:c65104-65047 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References