Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4549643..4550300 Replicon chromosome
Accession NZ_CP047677
Organism Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag GO012_RS21220 Protein ID WP_002916310.1
Coordinates 4549643..4550053 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag GO012_RS21225 Protein ID WP_002916312.1
Coordinates 4550034..4550300 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO012_RS21200 4545643..4547376 - 1734 WP_004149758.1 single-stranded-DNA-specific exonuclease RecJ -
GO012_RS21205 4547382..4548095 - 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GO012_RS21210 4548118..4549014 - 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
GO012_RS21215 4549115..4549636 + 522 WP_002916308.1 flavodoxin FldB -
GO012_RS21220 4549643..4550053 - 411 WP_002916310.1 protein YgfX Toxin
GO012_RS21225 4550034..4550300 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
GO012_RS21230 4550546..4551529 + 984 WP_014906951.1 tRNA-modifying protein YgfZ -
GO012_RS21235 4551628..4552287 - 660 WP_002916317.1 hemolysin III family protein -
GO012_RS21240 4552451..4552762 - 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
GO012_RS21245 4552812..4553540 + 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
GO012_RS21250 4553659..4555092 + 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T147227 WP_002916310.1 NZ_CP047677:c4550053-4549643 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T147227 NZ_CP062884:15394-15774 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT147227 WP_002916312.1 NZ_CP047677:c4550300-4550034 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT147227 NZ_CP062884:15173-15394 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References