Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1269697..1270316 Replicon chromosome
Accession NZ_CP047677
Organism Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVL1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GO012_RS05950 Protein ID WP_002892050.1
Coordinates 1269697..1269915 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GO012_RS05955 Protein ID WP_002892066.1
Coordinates 1269942..1270316 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO012_RS05920 1265744..1266007 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
GO012_RS05925 1266007..1266147 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GO012_RS05930 1266144..1266842 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
GO012_RS05935 1266943..1268394 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
GO012_RS05940 1268369..1268839 - 471 WP_002892026.1 YlaC family protein -
GO012_RS26625 1268860..1269000 + 141 WP_004147370.1 hypothetical protein -
GO012_RS05945 1268972..1269538 - 567 WP_002892030.1 maltose O-acetyltransferase -
GO012_RS05950 1269697..1269915 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GO012_RS05955 1269942..1270316 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GO012_RS05960 1270802..1273948 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GO012_RS05965 1273971..1275164 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T147217 WP_002892050.1 NZ_CP047677:c1269915-1269697 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T147217 NZ_CP062883:2021845-2021947 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT147217 WP_002892066.1 NZ_CP047677:c1270316-1269942 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT147217 NZ_CP062883:c2021985-2021714 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCACTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References