Detailed information of TA system TA147199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2643424..2645012
• Replicon: chromosome
• Accession: NZ_CP047675
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVF1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A331XLA5
• Locus tag: GO010_RS12375
• Protein ID: WP_014907613.1
• Coordinates: 2643690..2645012 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: GO010_RS12370
• Protein ID: WP_002904790.1
• Coordinates: 2643424..2643690 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO010_RS12345	2638602..2639525	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
GO010_RS12350	2639506..2640660	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
GO010_RS12355	2641006..2642385	+	1380	WP_004184021.1	purine permease	-
GO010_RS12360	2642363..2642863	+	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GO010_RS12365	2642860..2643186	+	327	WP_004215084.1	hydroxyisourate hydrolase	-
GO010_RS12370	2643424..2643690	+	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GO010_RS12375	2643690..2645012	+	1323	WP_014907613.1	type II toxin-antitoxin system HipA family toxin	Toxin
GO010_RS12380	2645029..2646321	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
GO010_RS12385	2646463..2647467	-	1005	WP_014907612.1	type 1 fimbrial protein	-
GO010_RS12390	2647455..2649956	-	2502	WP_014907611.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49139.47 Da
• Isoelectric Point: 7.5367

>T147199 WP_014907613.1 NZ_CP047675:2643690-2645012 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T147199 NZ_CP062879:57695-57982 [Escherichia coli O157:H16]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAAGAATGGATGAAACTGGATAAATCCATTCAGCAACAGTTCGC
AAAAAAATTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGAACTAAAAAATTGCTACAAAATCA
AACTTCGTGCATCGGGTTTTCGTCTGGTCTATCAGGTTATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAATAACGTTTATAATCTTGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT147199 WP_002904790.1 NZ_CP047675:2643424-2643690 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT147199 NZ_CP062879:57447-57698 [Escherichia coli O157:H16]
ATGCCTAATATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAATTGAAAAAAAACCCAATGGCAACAGTCGGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGAAACCAGCCGGCATTCTACTGTGTGCCTGCGGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGATCAGGAGCTGGTGAAACTGGTTACTGAACGTAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A331XLA5

Antitoxin

• AlphaFold DB: A0A0H3GXD8