Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 678254..679064 Replicon chromosome
Accession NZ_CP047675
Organism Klebsiella pneumoniae subsp. pneumoniae strain KUH-KPNHVF1

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag GO010_RS03140 Protein ID WP_014908042.1
Coordinates 678531..679064 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GO010_RS03135 Protein ID WP_002887278.1
Coordinates 678254..678520 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO010_RS03115 674126..674470 - 345 WP_012737331.1 cation efflux system protein CusF -
GO010_RS03120 674488..675873 - 1386 WP_012737332.1 efflux transporter outer membrane subunit -
GO010_RS03125 676045..676728 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
GO010_RS03130 676718..678151 + 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
GO010_RS03135 678254..678520 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
GO010_RS03140 678531..679064 + 534 WP_014908042.1 GNAT family N-acetyltransferase Toxin
GO010_RS03145 679112..680233 - 1122 WP_012737335.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T147197 WP_014908042.1 NZ_CP047675:678531-679064 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T147197 NZ_CP062879:2049-2534 [Escherichia coli O157:H16]
ATGGGATGTGTAACAGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTTCTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCTCTCGGAGCCACCCGGACTTTTGTTGTATGCAGGAAAGGTACGC
AACAAGTAGTCGGTTTTTACTCACTGGCTACTGGCAGTGTCAACCATACAGAAGCTACAGGCAATCTTCGGCGCAATATG
CCTGACCCCATCCCTGTCATTATACTTGCACGACTGGCTGTTGATGCGTCATTCCGTGGTAAAGGGCTTGGTGCGGATTT
ACTACATGATGCAGTTCGTCGTTGTTACAGAGTGGCCGAAAATATCGGTGTACGTGCAATTATGGTTCATGCACTTACGG
AAAGCGCAAAACAGTTTTATATCCATCATGGCTTTACACCATCAAAAACTCAGCTGCAGACATTGTTTCTTAAACTCCCT
CAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT147197 WP_002887278.1 NZ_CP047675:678254-678520 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT147197 NZ_CP062879:1777-2061 [Escherichia coli O157:H16]
ATGATATACGGAGGCTTTATGAAATCAGATGTTCAGCTCAACCTCAGAGCTAGAGAATCACAGCGGATACTCATTGATAC
CGCCGCAGAAATTCTTCATAAGTCACGTACAGACTTCATTCTTGAAATGGCCTGTAAAGCTGCGGAAGATGTTATTCTTG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCAGATGAGCCT
GCCATCGAAAAATTACTGGCAAGGAAACCTCAATGGGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References