Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 34715..35241 Replicon plasmid pCK41
Accession NZ_CP047672
Organism Edwardsiella piscicida strain CK41

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GT752_RS17590 Protein ID WP_000323025.1
Coordinates 34954..35241 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q5J3S4
Locus tag GT752_RS17585 Protein ID WP_000534857.1
Coordinates 34715..34954 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GT752_RS17555 (GT752_17380) 29883..30842 + 960 WP_000208727.1 DUF523 and DUF1722 domain-containing protein -
GT752_RS17560 (GT752_17385) 30988..32076 + 1089 WP_000633445.1 AAA family ATPase -
GT752_RS17565 (GT752_17390) 32139..32843 - 705 WP_001067852.1 IS6-like element IS26 family transposase -
GT752_RS17570 (GT752_17395) 33107..34180 - 1074 WP_000557466.1 hypothetical protein -
GT752_RS17575 34224..34373 - 150 WP_000550473.1 hypothetical protein -
GT752_RS17580 (GT752_17400) 34580..34690 - 111 Protein_38 hypothetical protein -
GT752_RS17585 (GT752_17405) 34715..34954 + 240 WP_000534857.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GT752_RS17590 (GT752_17410) 34954..35241 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GT752_RS17595 (GT752_17415) 35545..36327 - 783 WP_001531258.1 IS21-like element ISSen3 family helper ATPase IstB -
GT752_RS17600 (GT752_17420) 36324..37346 - 1023 WP_000627495.1 IS21-like element ISSen3 family transposase -
GT752_RS17605 (GT752_17425) 37485..37709 + 225 Protein_43 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / aph(3')-Ia - 1..86371 86371
- inside IScluster/Tn - - 32139..37346 5207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T147193 WP_000323025.1 NZ_CP047672:34954-35241 [Edwardsiella piscicida]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T147193 NZ_CP062876:4048308-4048643 [Escherichia coli O157:H16]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGTAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9085.55 Da        Isoelectric Point: 4.6820

>AT147193 WP_000534857.1 NZ_CP047672:34715-34954 [Edwardsiella piscicida]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL

Download         Length: 240 bp

>AT147193 NZ_CP062876:4048060-4048308 [Escherichia coli O157:H16]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE60

References