Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3838696..3839327 Replicon chromosome
Accession NZ_CP047669
Organism Klebsiella aerogenes strain HNHF1

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H3FL31
Locus tag GUU79_RS19905 Protein ID WP_015703741.1
Coordinates 3839052..3839327 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0H3FNK6
Locus tag GUU79_RS19900 Protein ID WP_015703740.1
Coordinates 3838696..3839055 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU79_RS19875 3834953..3835897 + 945 WP_015703735.1 nickel ABC transporter permease subunit NikB -
GUU79_RS19880 3835894..3836727 + 834 WP_015369278.1 nickel ABC transporter permease subunit NikC -
GUU79_RS19885 3836727..3837491 + 765 WP_015703737.1 nickel import ATP-binding protein NikD -
GUU79_RS19890 3837488..3838279 + 792 WP_032709345.1 nickel import ATP-binding protein NikE -
GUU79_RS19895 3838267..3838668 + 402 WP_032709347.1 nickel-responsive transcriptional regulator NikR -
GUU79_RS19900 3838696..3839055 - 360 WP_015703740.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GUU79_RS19905 3839052..3839327 - 276 WP_015703741.1 type II toxin-antitoxin system HicA family toxin Toxin
GUU79_RS19910 3839724..3840737 + 1014 WP_015369274.1 magnesium transporter -
GUU79_RS19915 3840778..3841971 - 1194 WP_015703742.1 NAD(P)/FAD-dependent oxidoreductase -
GUU79_RS19920 3842202..3843698 + 1497 WP_015369272.1 inorganic phosphate transporter PitA -
GUU79_RS19925 3843763..3844098 - 336 WP_015703743.1 universal stress protein UspB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10258.89 Da        Isoelectric Point: 10.4888

>T147178 WP_015703741.1 NZ_CP047669:c3839327-3839052 [Klebsiella aerogenes]
MEQQVLSLRKKQRNTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T147178 NZ_CP062876:107711-107965 [Escherichia coli O157:H16]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.02 Da        Isoelectric Point: 4.3472

>AT147178 WP_015703740.1 NZ_CP047669:c3839055-3838696 [Klebsiella aerogenes]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCREAGIEPYAQPEKLKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT147178 NZ_CP062876:107463-107714 [Escherichia coli O157:H16]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAAATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FL31

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FNK6

References