Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 226030..226649 Replicon chromosome
Accession NZ_CP047669
Organism Klebsiella aerogenes strain HNHF1

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag GUU79_RS02420 Protein ID WP_015367918.1
Coordinates 226030..226248 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag GUU79_RS02425 Protein ID WP_015367917.1
Coordinates 226275..226649 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU79_RS02390 222103..222363 + 261 WP_015367923.1 type B 50S ribosomal protein L31 -
GUU79_RS02395 222366..222506 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
GUU79_RS02400 222503..223213 - 711 WP_045361849.1 GNAT family N-acetyltransferase -
GUU79_RS02405 223316..224770 + 1455 WP_160184369.1 PLP-dependent aminotransferase family protein -
GUU79_RS02410 224745..225209 - 465 WP_015367920.1 YlaC family protein -
GUU79_RS02415 225335..225898 - 564 WP_015704609.1 maltose O-acetyltransferase -
GUU79_RS02420 226030..226248 - 219 WP_015367918.1 hemolysin expression modulator Hha Toxin
GUU79_RS02425 226275..226649 - 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
GUU79_RS02430 227121..230267 - 3147 WP_015704610.1 multidrug efflux RND transporter permease subunit -
GUU79_RS02435 230290..231489 - 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 217364..226649 9285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T147166 WP_015367918.1 NZ_CP047669:c226248-226030 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T147166 NZ_CP062871:3041764-3042075 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT147166 WP_015367917.1 NZ_CP047669:c226649-226275 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT147166 NZ_CP062871:3042076-3042366 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References