Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4426096..4426714 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GUU86_RS21170 Protein ID WP_001291435.1
Coordinates 4426096..4426314 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GUU86_RS21175 Protein ID WP_000344800.1
Coordinates 4426340..4426714 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS21135 4421385..4421957 + 573 WP_000779831.1 YbaY family lipoprotein -
GUU86_RS21140 4421988..4422299 - 312 WP_000409911.1 MGMT family protein -
GUU86_RS21150 4422678..4423031 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GUU86_RS21155 4423073..4424623 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GUU86_RS21160 4424787..4425257 - 471 WP_000136192.1 YlaC family protein -
GUU86_RS21165 4425373..4425924 - 552 WP_000102568.1 maltose O-acetyltransferase -
GUU86_RS21170 4426096..4426314 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GUU86_RS21175 4426340..4426714 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GUU86_RS21180 4427260..4430409 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GUU86_RS21185 4430432..4431625 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T147159 WP_001291435.1 NZ_CP047665:c4426314-4426096 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T147159 NZ_CP062871:c845667-845564 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT147159 WP_000344800.1 NZ_CP047665:c4426714-4426340 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT147159 NZ_CP062871:845526-845797 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ATGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References