147156

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 3786468..3787803 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag GUU86_RS18135 Protein ID WP_000342497.1
Coordinates 3786856..3787803 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A228Z6F8
Locus tag GUU86_RS18130 Protein ID WP_000152739.1
Coordinates 3786468..3786863 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS18115 3782540..3783706 - 1167 WP_001293040.1 restriction endonuclease subunit S -
GUU86_RS18120 3783703..3785259 - 1557 WP_000202816.1 type I restriction-modification system subunit M -
GUU86_RS18125 3785565..3786398 + 834 WP_001192741.1 DUF4942 domain-containing protein -
GUU86_RS18130 3786468..3786863 + 396 WP_000152739.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
GUU86_RS18135 3786856..3787803 + 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
GUU86_RS18140 3787841..3788026 - 186 WP_000066585.1 hypothetical protein -
GUU86_RS18145 3788405..3789055 + 651 WP_001037963.1 HNH endonuclease -
GUU86_RS18150 3789063..3790043 - 981 WP_000991446.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
GUU86_RS18155 3790108..3791214 - 1107 WP_001298927.1 N-acetylneuraminate epimerase -
GUU86_RS18160 3791234..3791950 - 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE / fimA / fimI / fimC 3765466..3797477 32011


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T147156 WP_000342497.1 NZ_CP047665:3786856-3787803 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T147156 NZ_CP062871:c100392-100285 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14605.90 Da        Isoelectric Point: 11.6915

>AT147156 WP_000152739.1 NZ_CP047665:3786468-3786863 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT147156 NZ_CP062871:100439-100505 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A228Z6F8

References