Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2845129..2845929 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag GUU86_RS13780 Protein ID WP_000342449.1
Coordinates 2845129..2845656 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GUU86_RS13785 Protein ID WP_001277108.1
Coordinates 2845663..2845929 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS13755 2840204..2840971 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GUU86_RS13760 2840968..2842245 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
GUU86_RS13765 2842242..2843168 - 927 WP_160183913.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GUU86_RS13770 2843216..2844325 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GUU86_RS13775 2844749..2845132 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
GUU86_RS13780 2845129..2845656 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
GUU86_RS13785 2845663..2845929 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
GUU86_RS13790 2846079..2847182 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GUU86_RS13795 2847453..2848307 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GUU86_RS13800 2848552..2849610 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
GUU86_RS13805 2849603..2850271 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T147152 WP_000342449.1 NZ_CP047665:c2845656-2845129 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T147152 NZ_CP062870:c113088-112930 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT147152 WP_001277108.1 NZ_CP047665:c2845929-2845663 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT147152 NZ_CP062870:c113351-113153 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References