Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2226869..2227523 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GUU86_RS10675 Protein ID WP_000244772.1
Coordinates 2226869..2227276 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GUU86_RS10680 Protein ID WP_000354046.1
Coordinates 2227257..2227523 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS10655 2222826..2224559 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
GUU86_RS10660 2224565..2225275 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GUU86_RS10665 2225300..2226196 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GUU86_RS10670 2226308..2226829 + 522 WP_001055874.1 flavodoxin FldB -
GUU86_RS10675 2226869..2227276 - 408 WP_000244772.1 protein YgfX Toxin
GUU86_RS10680 2227257..2227523 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GUU86_RS10685 2227766..2228746 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GUU86_RS10690 2228823..2229482 - 660 WP_000250274.1 hemolysin III family protein -
GUU86_RS10695 2229646..2229957 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
GUU86_RS10700 2230002..2231435 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
GUU86_RS10705 2231492..2232235 - 744 WP_032220047.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T147149 WP_000244772.1 NZ_CP047665:c2227276-2226869 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T147149 NZ_CP062870:c40464-40282 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT147149 WP_000354046.1 NZ_CP047665:c2227523-2227257 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT147149 NZ_CP062870:c40658-40461 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References