Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 732654..733292 Replicon chromosome
Accession NZ_CP047665
Organism Escherichia coli strain LD26-1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GUU86_RS03615 Protein ID WP_000813794.1
Coordinates 732654..732830 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GUU86_RS03620 Protein ID WP_001270286.1
Coordinates 732876..733292 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU86_RS03595 728273..729487 - 1215 WP_089078769.1 BenE family transporter YdcO -
GUU86_RS03600 729540..730076 + 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
GUU86_RS03605 730149..732110 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GUU86_RS03610 732202..732432 - 231 WP_000494244.1 YncJ family protein -
GUU86_RS03615 732654..732830 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GUU86_RS03620 732876..733292 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GUU86_RS03625 733371..734777 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
GUU86_RS03630 735022..736167 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
GUU86_RS03635 736185..737198 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GUU86_RS03640 737199..738140 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 726968..728233 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T147136 WP_000813794.1 NZ_CP047665:732654-732830 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T147136 NZ_CP062868:2629446-2629553 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT147136 WP_001270286.1 NZ_CP047665:732876-733292 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT147136 NZ_CP062868:c2629398-2629331 [Escherichia coli]
GTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References