Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 3414..3879 Replicon plasmid pLD93-1-MCR1
Accession NZ_CP047664
Organism Escherichia coli strain LD93-1

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag GUU84_RS23275 Protein ID WP_001230707.1
Coordinates 3628..3879 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag GUU84_RS23270 Protein ID WP_000801440.1
Coordinates 3414..3641 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU84_RS23245 459..962 - 504 WP_000781813.1 hypothetical protein -
GUU84_RS23250 1323..1670 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
GUU84_RS23255 1667..1915 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
GUU84_RS23260 2220..2570 - 351 WP_000854259.1 hypothetical protein -
GUU84_RS23265 2661..2960 - 300 WP_000650309.1 hypothetical protein -
GUU84_RS23270 3414..3641 - 228 WP_000801440.1 hypothetical protein Antitoxin
GUU84_RS23275 3628..3879 - 252 WP_001230707.1 hypothetical protein Toxin
GUU84_RS23280 3876..4418 - 543 WP_001083906.1 hypothetical protein -
GUU84_RS23285 4523..5038 - 516 WP_001025395.1 J domain-containing protein -
GUU84_RS23290 6453..7319 + 867 WP_001353743.1 replication initiation protein -
GUU84_RS23295 7332..7736 + 405 WP_012421203.1 hypothetical protein -
GUU84_RS23300 7871..8398 + 528 WP_000577043.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T147129 WP_001230707.1 NZ_CP047664:c3879-3628 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T147129 NZ_CP062868:c619594-619130 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT147129 WP_000801440.1 NZ_CP047664:c3641-3414 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT147129 NZ_CP062868:c619929-619594 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References