Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 67146..67747 Replicon plasmid pLD93-1-90kb
Accession NZ_CP047663
Organism Escherichia coli strain LD93-1

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag GUU84_RS23125 Protein ID WP_001216041.1
Coordinates 67367..67747 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag GUU84_RS23120 Protein ID WP_001190712.1
Coordinates 67146..67367 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU84_RS23070 62446..63135 + 690 WP_069067277.1 hypothetical protein -
GUU84_RS23075 63122..63808 + 687 WP_160188295.1 ead/Ea22-like family protein -
GUU84_RS23080 63805..64428 + 624 WP_160188296.1 ead/Ea22-like family protein -
GUU84_RS23085 64601..64789 + 189 WP_000797282.1 hypothetical protein -
GUU84_RS23090 64791..65000 + 210 WP_000951710.1 hypothetical protein -
GUU84_RS23095 64997..65698 + 702 WP_074446721.1 hypothetical protein -
GUU84_RS23100 65682..65966 + 285 WP_001142396.1 hypothetical protein -
GUU84_RS23105 65951..66160 - 210 WP_001344848.1 hypothetical protein -
GUU84_RS23110 66334..66585 + 252 WP_032220968.1 DNA polymerase III subunit theta -
GUU84_RS23115 66684..67073 + 390 WP_088491978.1 DNA repair protein -
GUU84_RS23120 67146..67367 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GUU84_RS23125 67367..67747 + 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
GUU84_RS23130 67831..68631 - 801 WP_000432093.1 hypothetical protein -
GUU84_RS23135 68638..69300 - 663 WP_160188297.1 DUF2829 domain-containing protein -
GUU84_RS23140 69315..69809 - 495 WP_000640903.1 dUTP diphosphatase -
GUU84_RS23145 69806..70282 - 477 WP_000861173.1 hypothetical protein -
GUU84_RS23150 70279..70587 - 309 WP_160188298.1 hypothetical protein -
GUU84_RS23155 70697..71725 - 1029 WP_121334858.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..90674 90674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T147127 WP_001216041.1 NZ_CP047663:67367-67747 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T147127 NZ_CP062868:163705-163857 [Escherichia coli]
ATGCCGCAGAAATATAGATTACTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGGGAGTTATGAGCTGGCGGCATTTTTAGCCTGCAATTTAAAAGAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT147127 WP_001190712.1 NZ_CP047663:67146-67367 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT147127 NZ_CP062868:c163654-163600 [Escherichia coli]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References