Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3749088..3749742 Replicon chromosome
Accession NZ_CP047662
Organism Escherichia coli strain LD93-1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GUU84_RS17770 Protein ID WP_000244777.1
Coordinates 3749088..3749495 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GUU84_RS17775 Protein ID WP_000354046.1
Coordinates 3749476..3749742 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU84_RS17750 3745045..3746778 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
GUU84_RS17755 3746784..3747494 - 711 WP_089570061.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GUU84_RS17760 3747519..3748415 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GUU84_RS17765 3748527..3749048 + 522 WP_001055874.1 flavodoxin FldB -
GUU84_RS17770 3749088..3749495 - 408 WP_000244777.1 toxin CptA Toxin
GUU84_RS17775 3749476..3749742 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GUU84_RS17780 3749985..3750965 + 981 WP_001514522.1 tRNA-modifying protein YgfZ -
GUU84_RS17785 3751161..3751820 - 660 WP_000250267.1 hemolysin III family protein -
GUU84_RS17790 3751984..3752295 - 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
GUU84_RS17795 3752340..3753773 + 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T147122 WP_000244777.1 NZ_CP047662:c3749495-3749088 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T147122 NZ_CP062867:c62915-62709 [Escherichia coli O39:H21]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT147122 WP_000354046.1 NZ_CP047662:c3749742-3749476 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT147122 NZ_CP062867:62902-62963 [Escherichia coli O39:H21]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References