Detailed information of TA system TA147121

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3695287..3695948
• Replicon: chromosome
• Accession: NZ_CP047662
• Organism: Escherichia coli strain LD93-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GUU84_RS17480
• Protein ID: WP_160188250.1
• Coordinates: 3695287..3695610 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: S3J179
• Locus tag: GUU84_RS17485
• Protein ID: WP_000065326.1
• Coordinates: 3695631..3695948 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU84_RS17455	3691135..3692364	+	1230	WP_000065950.1	amino acid permease	-
GUU84_RS17460	3692824..3693207	+	384	Protein_3404	helix-turn-helix transcriptional regulator	-
GUU84_RS17465	3693263..3693959	+	697	Protein_3405	IS1 family transposase	-
GUU84_RS17470	3694022..3694342	+	321	WP_001232281.1	hypothetical protein	-
GUU84_RS17475	3694376..3694582	-	207	WP_001570270.1	hypothetical protein	-
GUU84_RS17480	3695287..3695610	-	324	WP_160188250.1	TA system toxin CbtA family protein	Toxin
GUU84_RS17485	3695631..3695948	-	318	WP_000065326.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GUU84_RS17490	3695967..3696188	-	222	WP_000691995.1	DUF987 domain-containing protein	-
GUU84_RS17495	3696197..3696673	-	477	WP_000811694.1	RadC family protein	-
GUU84_RS17500	3696689..3697147	-	459	WP_016538084.1	antirestriction protein	-
GUU84_RS17505	3697233..3697469	-	237	WP_000004273.1	DUF905 domain-containing protein	-
GUU84_RS17510	3697547..3697957	-	411	WP_000912997.1	hypothetical protein	-
GUU84_RS17515	3698024..3698461	-	438	WP_024198335.1	hypothetical protein	-
GUU84_RS17520	3698503..3699039	-	537	WP_000219797.1	DUF4339 domain-containing protein	-
GUU84_RS17525	3699065..3699775	-	711	WP_024198336.1	hypothetical protein	-
GUU84_RS17530	3699984..3700808	-	825	WP_000197385.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3693263..3724411	31148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12320.36 Da
• Isoelectric Point: 9.6247

>T147121 WP_160188250.1 NZ_CP047662:c3695610-3695287 [Escherichia coli]
MKILPATISRAAKPCLPPVAVRQLLLTRLLEKHYGLTLNDTPFSDETVIKEHFDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTNIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T147121 NZ_CP062867:c59792-59505 [Escherichia coli O39:H21]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11830.50 Da
• Isoelectric Point: 6.9367

>AT147121 WP_000065326.1 NZ_CP047662:c3695948-3695631 [Escherichia coli]
MSNIIWGLQRDITPRLGTRLVQEGNQLHYLADRACITGKFSDAECLKLDVAFPHFISRMESMLSTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYVAVYSTQR

Nucleotide

• Length: 318 bp

>AT147121 NZ_CP062867:c60040-59789 [Escherichia coli O39:H21]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: S3J179