Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2289075..2290663 Replicon chromosome
Accession NZ_CP047662
Organism Escherichia coli strain LD93-1

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag GUU84_RS10795 Protein ID WP_001125439.1
Coordinates 2289075..2290397 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag GUU84_RS10800 Protein ID WP_001301023.1
Coordinates 2290397..2290663 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU84_RS23520 2284404..2285804 - 1401 WP_001409388.1 site-specific integrase -
GUU84_RS10775 2286097..2287494 + 1398 WP_025368664.1 ISNCY family transposase -
GUU84_RS10780 2287726..2287995 + 270 WP_001610304.1 hypothetical protein -
GUU84_RS10785 2287995..2288462 + 468 WP_025368663.1 hypothetical protein -
GUU84_RS10790 2288505..2288876 + 372 WP_001610302.1 hypothetical protein -
GUU84_RS10795 2289075..2290397 - 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
GUU84_RS10800 2290397..2290663 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
GUU84_RS10805 2290886..2292286 - 1401 WP_001083595.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2259678..2290663 30985
- flank IS/Tn - - 2286097..2287494 1397


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T147111 WP_001125439.1 NZ_CP047662:c2290397-2289075 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T147111 NZ_CP062865:c2700711-2700460 [Escherichia coli O39:H21]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT147111 WP_001301023.1 NZ_CP047662:c2290663-2290397 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT147111 NZ_CP062865:c2700463-2700209 [Escherichia coli O39:H21]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References