Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1098355..1098973 Replicon chromosome
Accession NZ_CP047662
Organism Escherichia coli strain LD93-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GUU84_RS05105 Protein ID WP_001291435.1
Coordinates 1098355..1098573 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GUU84_RS05110 Protein ID WP_000344800.1
Coordinates 1098599..1098973 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU84_RS05070 1093646..1094218 + 573 WP_000779831.1 YbaY family lipoprotein -
GUU84_RS05075 1094249..1094560 - 312 WP_000409911.1 MGMT family protein -
GUU84_RS05085 1094939..1095292 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GUU84_RS05090 1095334..1096884 - 1551 WP_001514985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GUU84_RS05095 1097048..1097518 - 471 WP_000136192.1 YlaC family protein -
GUU84_RS05100 1097633..1098184 - 552 WP_000102564.1 maltose O-acetyltransferase -
GUU84_RS05105 1098355..1098573 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GUU84_RS05110 1098599..1098973 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GUU84_RS05115 1099519..1102668 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GUU84_RS05120 1102691..1103884 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T147110 WP_001291435.1 NZ_CP047662:c1098573-1098355 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T147110 NZ_CP062865:c2700463-2700209 [Escherichia coli O39:H21]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT147110 WP_000344800.1 NZ_CP047662:c1098973-1098599 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT147110 NZ_CP062865:c2700711-2700460 [Escherichia coli O39:H21]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References