Detailed information of TA system TA147107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 636364..636914
• Replicon: chromosome
• Accession: NZ_CP047662
• Organism: Escherichia coli strain LD93-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: GUU84_RS02920
• Protein ID: WP_001160975.1
• Coordinates: 636600..636914 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D3H431
• Locus tag: GUU84_RS02915
• Protein ID: WP_000125565.1
• Coordinates: 636364..636597 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU84_RS02890	631435..631722	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
GUU84_RS02895	631780..633111	+	1332	WP_001183198.1	MFS transporter	-
GUU84_RS02900	633222..633752	+	531	WP_000600718.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
GUU84_RS02905	633745..635607	+	1863	WP_040234481.1	glutathione-regulated potassium-efflux system protein KefC	-
GUU84_RS02910	635799..636278	+	480	WP_001487721.1	type 3 dihydrofolate reductase	-
GUU84_RS02915	636364..636597	+	234	WP_000125565.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GUU84_RS02920	636600..636914	+	315	WP_001160975.1	CcdB family protein	Toxin
GUU84_RS02925	636911..637759	-	849	WP_021556397.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
GUU84_RS02930	637766..638143	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
GUU84_RS02935	638146..638967	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
GUU84_RS02940	638964..639953	-	990	WP_021556398.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GUU84_RS02945	639953..641239	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T147107 WP_001160975.1 NZ_CP047662:636600-636914 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T147107 NZ_CP062865:2093963-2094139 [Escherichia coli O39:H21]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8599.64 Da
• Isoelectric Point: 6.9791

>AT147107 WP_000125565.1 NZ_CP047662:636364-636597 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Nucleotide

• Length: 234 bp

>AT147107 NZ_CP062865:2094185-2094601 [Escherichia coli O39:H21]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A090NGC7