Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 326278..326775 Replicon chromosome
Accession NZ_CP047662
Organism Escherichia coli strain LD93-1

Toxin (Protein)


Gene name ghoT Uniprot ID A0A163EQM5
Locus tag GUU84_RS01470 Protein ID WP_001672275.1
Coordinates 326602..326775 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GUU84_RS01465 Protein ID WP_000398619.1
Coordinates 326278..326574 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU84_RS01445 323009..323728 - 720 WP_000611288.1 two-component system response regulator DcuR -
GUU84_RS01450 323725..325356 - 1632 WP_001216477.1 sensor histidine kinase -
GUU84_RS01455 325537..325767 + 231 WP_021557455.1 (4Fe-4S)-binding protein -
GUU84_RS01460 325779..326051 + 273 WP_000405647.1 N-acetyltransferase -
GUU84_RS01465 326278..326574 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GUU84_RS01470 326602..326775 + 174 WP_001672275.1 type V toxin-antitoxin system toxin GhoT Toxin
GUU84_RS01475 326894..328411 - 1518 WP_001295074.1 lysine--tRNA ligase -
GUU84_RS01480 328648..330105 - 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T147105 WP_001672275.1 NZ_CP047662:326602-326775 [Escherichia coli]
MALFSKILIFYVIGVNISFIIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T147105 NZ_CP062865:c1888329-1888222 [Escherichia coli O39:H21]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT147105 WP_000398619.1 NZ_CP047662:326278-326574 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT147105 NZ_CP062865:1888377-1888444 [Escherichia coli O39:H21]
GTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A163EQM5

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References